Pueraria Lobata and Pueraria Thomsonii for Mild Dyslipidemia: A Double-blinded Randomized Placebo-controlled Trial
Dyslipidemia is an important risk factor for atherosclerotic cardiovascular disease. Maintaining normal levels of lipid indicators can significantly reduce the risk of atherosclerotic cardiovascular disease and mortality. Empirical evidence suggests that adherence to Pueraria lobata and Pueraria thomsonii is useful for improving dyslipidemia, but evidence from randomized controlled trials is lacking. This randomized, double-blind controlled trial is therefore designed to evaluate the efficacy and safety of Pueraria lobata and Pueraria thomsonii for dyslipidemia.
Using radix pueraria flavonoids (RPFs) as a reducing and stabilizing agent, we report a simple, cost-effective, and ecologically friendly green synthesis technique for gold nanoparticles (AuNPs) in the present study. Ultraviolet-visible (UV) spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) investigations were used to characterize the AuNPs. The results demonstrated that the produced AuNPs were nearly spherical and that their particle sizes had a mean diameter of 4.85 ± 0.75 nm. The "Green" AuNPs, exhibiting remarkable peroxidase-like activity and Michaelis-Menten kinetics with high affinity for H2O2 and 3,3',5,5'-tetramethylbenzidine (TMB), were effectively applied to the fabrication of a sensitive nonenzymatic enhanced electrochemical sensor for the detection of cholesterol (Cho). Under optimum circumstances, it was possible to establish two linear ranges of 1-100 and 250-5000 μmol/L with a detection limit of 0.259 μmol/L (signal/noise ratio (S/N) = 3). The suggested sensor was utilized with satisfactory findings to determine the amount of Cho in food samples.
2019-08-01·Nanomedicine (London, England)3区 · 医学
Improved brain delivery of pueraria flavones via intranasal administration of borneol-modified solid lipid nanoparticles.
3区 · 医学
作者: Liping Wang ; Xiao Zhao ; Junfeng Du ; Mei Liu ; Jianfang Feng ; Kaili Hu
Aim: To improve the drug delivery to the brain with borneol (Bo)-modified solid lipid nanoparticles (SLNs) of pueraria flavones (PTF) via intranasal administration. Materials & methods: PTF-loaded SLNs were modified with Bo by physical and chemical methods to synthesize PTF-Bo-SA-SLNs and PTF-Bo-SLNs. The prepared SLNs were characterized and their brain delivery effects were evaluated in vitro and in vivo. Results: There was a more pronounced accumulation of PTF-Bo-SA-SLNs in Caco-2 cells. Following intranasal administration, more coumarin-6 was found in the rat brain carried by Bo-SA-SLNs. Brain area under the curve and Cmax of PTF-Bo-SA-SLN were 7.31- and 7.29-times higher than those of PTF-SLN, respectively. Conclusion: PTF-Bo-SA-SLNs are a promising therapeutic carrier for brain disease after intranasal administration.
2018-06-01·Journal of Separation Science3区 · 化学
Development of a liquid chromatography-tandem mass spectrometry method for simultaneous determination of five isoflavonoids and seven neurochemicals in rat brain dialysate and its application to a pharmacological study
Pueraria lobata is a medicinal plant widely used in traditional Chinese medicine. The total pueraria isoflavones have demonstrated positive effect against neurological disorders. In the present study, we first develop an ultra high performance liquid chromatography and tandem mass spectrometry method to quantify the multiple active pueraria isoflavonoids and neurochemical markers in brain dialysate to provide tools for further exploring the functional mechanism of pueraria isoflavones for neuroactivities. A phenomenex Luna C18 column (50 × 2.0 mm, 5 μm) was employed with acetonitrile/0.05% formic acid in water as the mobile phase for the separation of analytes. A mass spectrometer with electrospray ionization source in positive/negative ion switching mode was used for multiple reaction monitoring of the detected compounds. The method was validated and proved acceptable. The intra- and interday precision across quality control levels was within 13.87 for all analytes, whereas the deviation of assay accuracies ranged between 0.03 and 11.53%. The method was successfully applied to a pharmacological study of pueraria isoflavones in rat brain.