BACKGROUNDNon-vital tooth discoloration is a common condition in dental practice. The aim of this in vitro study was to evaluate the efficacy of intracoronal bleaching treatments of non-vital teeth either activated or not by Er,Cr:YSGG laser (2780 nm) operating at 1.25 or 2.5 W.METHODSTwenty four human canines were artificially stained after implementation of root canal treatment. Subsequently, the teeth were randomly divided into three groups (n = 8): Group 1 (control) received intracoronal bleaching treatment with a bleaching gel containing 35 % hydrogen peroxide for 40 min, Group 2 received the same treatment assisted with Er,Cr:YSGG laser (2780 nm) operating at 1.25 W average power for 30 s and Group 3 received the same treatment with Group 2, but the average power was adjusted to 2.5 W. The bleaching treatments repeated after one week. Spectrophotometric analysis of tooth color change (ΔE) was implemented 7 days after both bleaching sessions.RESULTSThe results of two-way ANOVA revealed that there was a tendency of no significant difference in color change between the three experimental groups (p = 0.063). However, the between-group comparisons showed that laser 2.5 W group had significantly higher ΔE than the control group after the first bleaching session. Moreover, a significant interaction between bleaching treatment and number of applications was detected (p = 0.026).CONCLUSIONSEr,Cr:YSGG laser irradiation significantly increased ΔE only after the first bleaching session when operating at 2.5 W. After the second bleaching session ΔE was not different compared to the control group, irrespectively of the laser power settings.