Immunization programs have implemented live attenuated mumps vaccines which reduced mumps incidence ≥97%. Some of the vaccine strains were abandoned due to unwanted side effects and the genetic marker of attenuation has not been identified so far. Our hypothesis was that non-infectious viral particles, in particular defective interfering particles (DIPs), contribute to neuroattenuation. We showed that non-infectious particles of the mumps vaccine L-Zagreb attenuated neurovirulence of wild type mumps virus 9218/Zg98. Then, we attenuated recent wild type mumps virus MuVi/Zagreb.HRV/28.12 in Vero cells through 16 passages but already the fifth passage (p5) showed accumulation of DIPs and attenuated neurovirulence in a newborn rat model when compared to the second passage (p2). Sequence analysis of the p2 and p5 revealed a single mutation in the 5' untranslated region of the HN gene. Analysis of the expression level of the HN protein showed that this mutation does not affect the expression of the protein. We conclude that the passages of MuVi/Zagreb.HRV/28.12 in Vero cells for only three passages accumulated DIPs which attenuate neurovirulence. These findings reveal DIPs as a very promising and general neuroattenuating factor which should be considered in the rational design of the new mumps vaccine.

1971-07-01·Canadian Journal of Microbiology4区 · 生物学

A field trial with live attenuated mumps virus vaccine: a serological study

4区 · 生物学

Article

作者: Marchessault, V. ; Rossier, E. ; Meunier, J. ; Baker, F.

A controlled study with live attenuated mumps virus vaccine, B level Jeryl Lynn strain, was conducted on a group of mentally retarded patients, children and adults; 159 of them were studied, and 109 received the vaccine. The follow-up consisted of clinical observation, temperature recording in both vaccinees and controls for 1 week after vaccine administration, and serological assay by complement fixation and neutralization at the time of vaccination, at 1 month and at [Formula: see text] months.There was no statistical evidence of temperature rise in the vaccinees as compared with the controls nor any untoward clinical reactions. At [Formula: see text] months, the seroconversion rate was 48.8% when assayed by complement-fixation tests, and 90% when assayed by neutralization tests. It was concluded that the vaccine was safe and potent.

1970-06-01·Biken journal

Studies on live attenuated mumps virus vaccine. 1. Attenuation of mumps virus by serial passage in the chorioallantoic cavity of developing chick embryos and field trials by the inhalation method.

Article

作者: Yamanishi, K ; Ogino, T ; Ueda, S ; Hosai, H ; Minekawa, Y

100 项与腮腺炎减毒活疫苗(深圳康泰生物制品) 相关的药物交易

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适应症	最高研发状态	国家/地区	公司	日期
腮腺炎	临床前	美国	深圳康泰生物制品股份有限公司	2023-05-23
腮腺炎	临床前	中国	深圳康泰生物制品股份有限公司	2023-05-23