Comparison of five retinoids for anti-photoaging therapy: Evaluation of anti-inflammatory and anti-oxidative activities in vitro and therapeutic efficacy in vivo

作者: Yuan, Zhengqiang ; Jiang, Ling ; Shu, Peng ; Li, Menggeng ; Wen, Ju ; Lin, Li ; Li, Yi ; Aisa, Haji Akber ; Du, Zhiyun

Over the past decades, increasing evidences have demonstrated that five retinoids, including retinol (ROL), retinol acetate (RAc), retinol propionate (RP), retinol palmitate (RPalm), and hydroxypinacolone retinoate (HPR), can be potential therapeutic agents for skin photoaging.However, therapeutic efficacies and biosafety have never been compared to these compoundsThis study aimed to determine the optimal retinoid type(s) for anti-photoaging therapy both in vitro and in vivo.Our data demonstrated that four retinoids (RPalm, RP, HPR and ROL) but not RAc were effective for anti-photoaging treatment at 5μg/mL in vitro, with action mechanisms associated with antioxidative, anti-inflammatory and anti-skin ECM degradation activities.Notably, both RPalm and RP appeared superior to HPR and ROL for those activities.Importantly, both RPalm and RP were shown to be optimal for anti-photoaging therapy when topically applied at 5 mg/kg in a UVB-induced mice model of photoaging, which is consistent with their high anti-photoaging activities in vitro.Addnl., topical application of these five retinoids showed satisfactory biosafety without causing significant apoptosis in animal organs, although RP application led to a slight decline in animal body weightsCollectively, these data have laid a good foundation for the next development of the clin. application of these retinoids for skin healthcare.

Current Issues in Molecular Biology

Improvement in Facial Wrinkles Using Materials Enhancing PPARGC1B Expression Related to Mitochondrial Function

Article

作者: Kang, Nae-Gyu ; Ye, Sanghyun ; Lee, Hyejin ; Kim, Juhyun ; Jun, Seung-Hyun

Skin aging is an unavoidable natural phenomenon caused by intrinsic and extrinsic factors. In modern society, the pursuit of a wrinkle-free and aesthetically appealing face has gained considerable prominence. Numerous studies have aimed at mitigating the appearance of facial wrinkles. Antiaging research focused on regulating the function of mitochondria, the main reactive oxygen species-generating organelles, has been extensively conducted. In this study, we investigated the correlation between facial wrinkles and the expression of PPARGC1B, considering the association of this gene with mitochondrial function, to identify its potential as a target for exploring antiaging cosmetic materials. We elucidated the role of PPARGC1B in the skin and identified five bioactive materials that modulated its expression. The effectiveness of these materials was verified through in vitro experiments on human dermal fibroblasts. We prepared cosmetic formulations incorporating the five materials and confirmed their ability to enhance dermal collagen in three-dimensional skin models and reduce facial wrinkles under the eyes and nasolabial fold areas in human subjects. The study findings have significant implications for developing novel antiaging cosmetic formulations by reinforcing mitochondrial functions.

Riyong Huaxue Gongye

Determination of 7 retinol and its derivatives in raw materials and cosmetics by high performance liquid chromatography

作者: Yan, Xiufang ; Chen, Yan ; Yan, Xiaojuan

An anal. method for the determination of retinol and its six derivatives (retinal, retinyl acetate, retinyl propionate, retinyl palmitate, hydroxypinacolone retinoate and retinyl retinoate) in raw materials and cosmetics has been developed.The sample was extracted with acetonitrile, followed by high performance liquid chromatog. (HPLC) determination with diode array detector (DAD), then it was separated by ZORBAX Eclipse Plus C-18 (250 mm×4.6 mm, 5 μm) using acetonitrile and THF as the mobile phase with gradient elution.The column temperature was 30°C, and the flow rate was 1 mL/min.The diode array detector was used to detect seven kinds of retinols and their derivatives in raw materials and cosmetics at multi-wavelength.The method is successfully validated which shows a good linearity (0.9997-1) with the limits of detection in the range of 0.5-2 μg/mL and quantification ranged from 1.5 to 6 μg/mL, resp.Finally, the proposed anal. method is applied to the determination of retinol and its six derivatives in different dosage forms of cosmetic samples containing essence, emulsion and cream, with good relative recovery values (92.37%-112.06%), and the precision (RSD) ranged from 0.20% to 6.14%.Thus, the results show that matrix effects are negligible.The method shows a good practicability and reproducibility through detection of 14 batches of samples.The good anal. features of the proposed method besides its simplicity and affordability, make it useful to carry out the quality control of cosmetic products.The establishment of this method is helpful for cosmetic enterprises to search for high-quality raw materials, control the best balance between efficacy and measurement, verify whether the production process is reliable and ensure the stability of raw materials and product efficacy.At the same time, it provides reliable quality inspection means for enterprises when developing and producing products.This method not only meets the simultaneous determination of multiple components in batch samples, but also improves the detection efficiency and reduces the exptl. cost.The establishment of this test method is of great significance for the market supervision of cosmetics and cosmetic raw materials.

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适应症	最高研发状态	国家/地区	公司	日期
寻常痤疮	临床1期	美国	Molecular Design International, Inc.	-
光线性皮肤障害	临床1期	美国	Molecular Design International, Inc.	-
银屑病	临床1期	美国	Molecular Design International, Inc.	-