BACKGROUNDPorcine epidemic diarrhea virus (PEDV) is an infectious, highly contagious virus, and is an etiological agent of acute entero-pathogenic diarrhea in swine.OBJECTIVESEvaluation of the antibody response of two types of PEDV vaccines is to be carried out.ANIMALS AND METHODSSows were vaccinated with either live or killed commercial PEDV SM98 (GenBank: GU937797.1) vaccines. Four different groups of sows with five sows in each group were used in this study: the unvaccinated negative control group, the killed virus vaccination group with killed virus boosting (K/K), the live virus vaccinated group with live virus boosting (L/L), and the combination group vaccinated with live virus and subsequently boosted with killed vaccine (L/K). Sows were vaccinated intramuscularly twice at four and two weeks prior to farrowing with 2ml/head vaccine dose. Antibody titers in sow and piglet serum one week after farrowing and that in colostrum were compared by enzyme-linked immunosorbent assay (ELISA) and serum neutralization test.RESULTSVaccination with K/K vaccine induced the highest level of IgG and IgA in sow serum, colostrum, and especially in piglet serum, with the lowest levels found in the L/L group. The major neutralizing activity was also found in the K/K group, particularly in colostrum, with piglets bearing higher neutralizing activity compared to sow sera. Among recombinant spike S1, S2, S3, and nucleocapsid N protein of PEDV, S3 protein presented the highest antibody level in the K/K group.CONCLUSIONKilled PEDV SM98 vaccine induced higher antibody levels.CLINICAL IMPORTANCEThis study clearly confirms that killed vaccine has induced higher antibody levels and may contribute to the design of future research and vaccine programs.

1985-01-01·Veterinarno-meditsinski nauki

Promishlen metod ze aerogenno imunizirane na praseta protiv chuma.

Article

作者: Petrova-Rafailova, A ; Tabakov, T ; Dimitrov, K ; Zlatev, D ; Gavrilov, N

An industrial method is presented through a model with a chamber for aerogenic immunization of about 250 pigs, aged 2.5 months. The experiments were carried out with more than 1,000 pigs of the Camborough hybrid under industrial conditions of raising. The process of immunization was feasibly carried out when the pigs passed through the chamber using aerosol at mixing them upon transference from the growing section to the fattening one, according to the provisions of the raising technology. A lapinized K-vaccine against fever, obtained from rabbits or tissue cultures was used in a dispersed state. Essential proved the high titer of the vaccine. Used were the aerosol devices DAG-2 or Microjet Fogmaster mounted at 1.5 m above the chamber floor. They guarantee the production of highly dispersed aerosol of an average arithmetic size of 7-10 microns, its concentration reaching 3,000,000 particles per 1 cm3. The animals were exposed for 30 min with Fogmaster and 45 min with DAG-2. It was shown that they tolerated perfectly this group immunization. A single procedure with the vaccine conferred immunity at 2.10(5) LT50 and protected the pigs from fever over the entire fattening period. The method was evaluated as a biotechnology.

1984-02-01·Journal of Clinical Microbiology2区 · 医学

Escherichia coli K5 antigen in relation to various infections and in healthy individuals

2区 · 医学

Article

作者: Jodal, U ; Kaijser, B

A phage specific for Escherichia coli K5 antigen was used to determine the frequency of K5 in strains of E. coli isolated from cases of sepsis, meningitis, and urinary tract infection (pyelonephritis, cystitis, and asymptomatic bacteriuria), as well as from fecal samples of healthy subjects. Although entirely absent from meningitic strains, K5 was found to be one of the most common E. coli capsular antigens, especially in strains causing sepsis. K5 was less common in the fecal strains of healthy subjects than in strains causing various urinary tract infections, between which there was no significant difference in its frequency. Thus, even if K5 is a less important antigen than K1 in the etiology of acute pyelonephritis or acute meningitis, as the fifth most commonly occurring K antigen in E. coli strains causing urinary tract infection it should be included as a component in any K antigen-based vaccine against acute pyelonephritis.

100 项与 K-Ras based vaccine(ASCENDO Biotechnology) 相关的药物交易

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