Bifidobacterium bifidum Strain BB1 Inhibits Tumor Necrosis Factor-α–Induced Increase in Intestinal Epithelial Tight Junction Permeability via Toll-Like Receptor-2/Toll-Like Receptor-6 Receptor Complex–Dependent Stimulation of Peroxisome Proliferator-Activated Receptor γ and Suppression of NF-κB p65

Article

作者: Abdulqadir, Raz ; Haque, Mohammad ; Gupta, Yash ; Al-Sadi, Rana ; Rawat, Manmeet ; Ma, Thomas Y

Bifidobacterium bifidum strain BB1 causes a strain-specific enhancement in intestinal epithelial tight junction (TJ) barrier. Tumor necrosis factor (TNF)-α induces an increase in intestinal epithelial TJ permeability and promotes intestinal inflammation. The major purpose of this study was to delineate the protective effect of BB1 against the TNF-α-induced increase in intestinal TJ permeability and to unravel the intracellular mechanisms involved. Previously reported, TNF-α produces an increase in intestinal epithelial TJ permeability in Caco-2 monolayers and in mice. The addition of BB1 inhibited the TNF-α increase in Caco-2 intestinal TJ permeability and mouse intestinal permeability in a strain-specific manner. BB1 inhibited the TNF-α-induced increase in intestinal TJ permeability by interfering the with TNF-α-induced enterocyte NF-κB p50/p65 and myosin light chain kinase (MLCK) gene activation. The BB1 protective effect against the TNF-α-induced increase in intestinal permeability was mediated by toll-like receptor-2/toll-like receptor-6 heterodimer complex activation of peroxisome proliferator-activated receptor γ (PPAR-γ) and PPAR-γ pathway inhibition of TNF-α-induced IKK-α activation, which, in turn, resulted in a step-wise inhibition of NF-κB p50/p65, MLCK gene, MLCK kinase activity, and MLCK-induced opening of the TJ barrier. In conclusion, these studies unravel novel intracellular mechanisms of BB1 protection against the TNF-α-induced increase in intestinal TJ permeability. Our data show that BB1 protects against the TNF-α-induced increase in intestinal epithelial TJ permeability via a PPAR-γ-dependent inhibition of NF-κB p50/p65 and MLCK gene activation.

2024-08-01·European Journal of Sport Science

A kinetic analysis of four high velocity, horizontally focused step‐up variations for acceleration training

Article

作者: Wade, Jarrod A. ; Glassbrook, Daniel J. ; Fuller, Joel T. ; Dorman, Chris A. ; Doyle, Tim L.A.

AbstractStep‐up variations are frequently used in sports performance to develop coordinated and powerful movements that transfer to running. This study aimed to quantify the kinetic characteristics of the first foot contact of four different step‐up variations. Ten professional rugby league players participated in this study and performed the Barbell One Box Step‐Up with Catch (BB1), Barbell Two Box Step‐Up (BB2), Vest Two Box Run (VEST) and Step‐Up Jump (JUMP) as part of routine in‐season strength training sessions during one season. Peak force, total impulse and maximal rate of force development (RFD) were measured from first foot contact on the step‐up box. Significantly greater peak force and RFD were observed in JUMP than any other variation (standardized mean difference; SMD: 3.9–5.5; p < 0.001). Total impulse was equal between JUMP and BB1, and significantly greater in JUMP than BB2 and VEST (SMD: 1.3–2.3; p < 0.001), and in BB1 than BB2 and VEST (SMD: 1.8–2.8; p < 0.001). Significantly larger peak force and RFD were observed in BB2 and VEST than BB1 (SMD: 0.6–0.7) and in total impulse in BB2 than VEST (SMD: 1.6) (p < 0.05). The results of this study highlight that step‐up exercise variations maximize different kinetic characteristics, which may transfer differently to athlete running performance.

2024-01-30·ACS Omega

Metal Oxide-Impregnated Biochar for Azo Dye Remediation as Revealed through Kinetics, Thermodynamics, and Response Surface Methodology

Article

作者: Aslam, Adeel ; Mustaqeem, Muhammad ; Noreen, Sobia ; Batool, Fozia ; Albalawi, Bedur Faleh A ; Abdelrahman, Ehab A ; Ditta, Allah

This study reports for the first time the adsorption capacity of a novel adsorbent Croton bonplandianus Baill. biochar. Its adsorption capacity was further enhanced by loading magnetic composites on it, which makes it an efficient medium for the adsorption of dyes. Two azo dyes, Basic Brown 1 (BB₁) and Basic Orange 2 (BO₂), were studied for their effective adsorption from aqueous media. A comprehensive characterization was performed by using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) to study the properties of Fe₂O₃-loaded C. bonplandianus Baill. biochar (FO-CBPBB). A series of batch experiments were conducted to optimize various parameters (pH, contact time, adsorbent amount, initial BB₁ and BO₂ concentrations, and temperature) for the maximum adsorption of BB₁ and BO₂ on the FO-CBPBB adsorbent. The percentage of BB₁ and BO₂ dyes that adsorb to FO-CBPBB under the best experimental circumstances (pH of solution 7, contact time 80 min, temperature of solution 40 °C, initial BB₁ and BO₂ dye concentrations 80 mg L^-1, and adsorbent dose 1 g L^-1) was 93 and 95%, respectively. The best adsorption of BB₁ and BO₂ was accomplished by optimizing the effects of several factors, including the starting dye concentration, contact time, and temperature, based on the central composite design. The Freundlich and Langmuir isotherm models were used to examine the equilibrium data. The Langmuir isotherm with the greatest adsorption capacity and R² value effectively captured the experimental results. When kinetic parameters were investigated, it was found that pseudo-second-order was appropriate, reflecting the fact that the dye-adsorbent interaction was the rate-controlling factor in this study. The sorption process was endothermic and spontaneous, as shown by the thermodynamic variables. Based on the interaction between the adsorbent and azo dyes, it was concluded that the adsorption process was electrostatic in nature. Adsorbents that have been synthesized can effectively remove azo dyes from wastewater. Excellent regeneration efficiency was exhibited by FO-CBPBB, which makes it an eco-friendly and cost-effective alternative to other costly techniques applied for water purification.

100 项与 BB-1 相关的药物交易

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