The rapid and non-destructive quantification of catechins in fermented black tea is crucial for evaluating the quality of black tea. The combination of hyperspectral imaging and chemometrics has been applied for quantitative detection, but its performance is usually constrained by the limited dataset size. Targeted at the challenge of insufficient samples in regression analysis of catechins, this study proposes an improved deep convolutional generative adversarial network with labeling module, named as DCGAN-L for hyperspectral data augmentation. The DCGAN-L consists of the spectral and label generating modules. First the synthetic spectra were generated, and an indicator was proposed to evaluate their quality. Then, the corresponding label values were generated, including epicatechin gallate (ECG), epicatechin (EGC), catechin (C), and total catechin (CC). For label generating, the Euclidean distances between the synthetic spectrum and all true spectra were measured, followed by allocating weights for calculating the label values based on these distances. Subsequently, the training dataset was augmented with the generated synthetic data. The effect of data augmentation was finally evaluated based on two regression models of random forest (RF) and broad learning system (BLS) for the quantification of catechins. Compared with the results before data augmentation, the average R² of RF and BLS models increased by 0.044 and 0.164, respectively. The proposed DCGAN-L model allows for the rapid, non-destructive quantification of catechins in black tea in the case of limited sample size.

2024-12-01·Plant, Cell & Environment

DkDTX1/MATE1 mediates the accumulation of proanthocyanidin and affects astringency in persimmon

Article

作者: Liu, Ying ; Xu, Liqing ; Sun, Chenfeng ; Zhang, Qinglin ; Wu, Xin ; Chen, Wenxing ; Luo, Zhengrong

AbstractProanthocyanidins (PAs) is a kind of polyphenols widely distributed in plants, and their astringent properties can protect plants from herbivores and regulate fruit taste. There is a great difference in PA composition between astringent (A)‐type and nonastringent (NA)‐type persimmon. Here, we studied the potential of DkDTX1/MATE1 in regulating PAs composition through its preferred transport in persimmon fruit. The results of fluorescence microscope showed that the DkDTX1/MATE1 green fluorescence overlapped with the blue light emitted by PA. Overexpression of DkDTX1/MATE1 in persimmon leaves not only significantly increase the concentrations of PA, but also upregulated the expression of PA biosynthesis pathway genes. Further overexpression of DkDTX1/MATE1 in persimmon fruit discs and stable genetic transformation of DkDTX1/MATE1 also led to PA concentrations increased. Molecular docking and transporter assays showed that DkDTX1/MATE1 preferentially transported catechin, epicatechin gallate and epigallocatechin gallate. DkDTX1/MATE1 mainly bound to the PA precursors via serine at position 68. Our findings indicate that DkDTX1/MATE1 play a role in the accumulation of PAs in early stage of fruit development and affects the astringency of persimmon through preferential transport PA precursors, which provided a theoretical basis for the future use of metabolic engineering to regulate the composition of PAs in persimmon.

2024-10-04·Mini-Reviews in Medicinal Chemistry

Exploring the Therapeutic Potential of Green Tea (Camellia sinensis L.) in Anti-Aging: A Comprehensive Review of Mechanisms and Findings

Article

作者: Sivamaruthi, Bhagavathi Sundaram ; Wang, Shucai ; Kesika, Periyanaina ; Chaiyasut, Chaiyavat ; Sisubalan, Natarajan

Green tea (GT) is rich in Phyto-active compounds such as epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG), epicatechin (EC), catechin, and tannic acid, which exhibit synergistic effects when combined. Preclinical studies demonstrate that GT and its compounds can reduce reactive oxygen species (ROS), enhance antioxidant capacity, and alleviate aging-related issues such as memory impairments, cognitive decline, and shortened lifespan. Clinical trials corroborate the efficacy of topical GT formulations in improving skin tone, texture, and elasticity and reducing wrinkles. The present manuscript summarizes the recent update on the anti-aging potential of GT and its possible mechanisms. The literature survey suggested that GT consumption is linked to improved cognition, reduced depression levels, and activation of pathways in model organisms like C. elegans. Additionally, tea polyphenols enhance fibroblast mitophagy, boost hippocampal synaptic plasticity in rodents, and mitigate age-related cognitive decline. Moreover, EGCG exhibits anti-aging properties by reducing TNF-induced MMP-1 expression, suppressing ERK signaling, and inhibiting MEK and Src phosphorylation in human dermal fibroblasts. In the context of skin permeation and deposition, optimized transpersonal formulation (TF) incorporating EGCG and hyaluronic acid (HA) demonstrated significantly increased skin permeation and deposition of EGCG compared to plain EGCG. Furthermore, EGCG protects cardiomyocytes via the PPARγ pathway and combats age-related muscle loss through miRNA-486-5p regulation, AKT activation, and FoxO1a-mediated expression of MuRF1 and Atrogin-1. In conclusion, the regular consumption of GT holds promise for promoting physical and mental health, delaying brain and skin aging, and improving overall health by enhancing total antioxidant capacity.

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适应症	最高研发状态	国家/地区	公司	日期
动脉粥样硬化	临床前	中国	西安交通大学	2024-09-01
先天性心脏缺损	临床前	-	Landeskrankenhaus -Anstalt des öffentlichen Rechts	2024-05-18