SARS-CoV-2 trivalent vaccine (CHO Cell)(Guangzhou Patronus Biotech)

The murine B-cell differentiation antigen Lyb-2 has been shown to be involved in B-lymphocyte activation and has been postulated by some to be related to a receptor for B-cell stimulatory factor I (BSF-1) (H. Yakura et al., J. Immunol. 137, 1475, 1986). Here we have demonstrated that monoclonal antibody (mAB) to Lyb-2 resembles BSF-1 in its ability to activate small resting B cells and enhancement of surface Ia. Anti-Lyb-2 antibodies bound B cells with very high avidity and were able to induce mobilization of cytosolic-free calcium. Anti-Lyb-2 mAB differs from BSF-1 in that BSF-1 but not anti-Lyb-2 is able to synergize with anti-mu in induction of B-cell proliferation. The relation between Lyb-2 molecules and BSF-1 receptors was tested in assays that measure binding of anti-Lyb-2 or BSF-1 in B cells and were found not to compete with each other. It appears that the two B-cell agonists anti-Lyb-2 and BSF-1 may exert their effects on B cells through different cell surface moieties as well as different intracellular pathways.

Panax notoginseng (Burk.) F. H. Chen, a valuable Chinese herb in Yunnan Province, is highly susceptible to leaf spot disease caused by Boeremia linicola. However, effective control methods for leaf spot in P. notoginseng remain limited. We surveyed understory P. notoginseng populations in Lincang, Lancang, and Xundian, where the incidence rate reached up to 80%, and the disease index was as high as 25 in severe cases. We found that B. linicola can be transmitted through the air and overwinter in infected leaves and soil, with conidia and pycnidia serving as the primary infection source. The optimum growth conditions for the in vitro culture of the pathogenic isolate B. linicola LYB-2 were 20°C and pH 5.0. We also screened potential control agents using plate and colony-inhibition assays. Among the tested agents, tetramycin exhibited 38.56 to 74.67% inhibition against LYB-2, with a half-maximal effective concentration (EC 50 ) of 0.60 mg/liter. Trichoderma atroviride and T. harzianum exhibited inhibition rates of 52.48 and 44.19%, respectively, while Bacillus subtilis reached 78.45%. Field tests demonstrated significant inhibitory effects of B. subtilis, T. atroviride BH-10 preparation, and 0.3% tetramycin, achieving 72.56, 71.3, and 70.2% inhibition, respectively. This study provides a theoretical foundation for ecofriendly management of P. notoginseng leaf spot disease while also establishing a critical scientific basis for disease prediction and sustainable control strategies.