In the present study, the effectiveness of atmospheric pressure cold plasma (APCP) in reducing aflatoxins (AFB1, AFB2, AFG1, and AFG2) and total aflatoxin in naturally contaminated and spiked mixed nuts as well as in standard toxin solutions was investigated. According to the results, after 16 min of APCP treatment, AFB1 levels decreased by 24.6 % in naturally contaminated nuts and by 88.4 % in spiked samples, while an 80.9 % reduction was observed in standard toxin solutions after just 2 min. The total aflatoxin levels decreased by 22.7 % in naturally contaminated samples within 16 min, while this rate was 72.6 % in spiked samples. Considering the standard toxin solution, a rapid 76.3 % decrease was observed in total aflatoxin level in just 2 min. While APCP slightly affected moisture, free fatty acid, and peroxide values, other quality parameters remained stable. These results demonstrate that mycotoxin form and food matrix significantly influence detoxification efficiency.