作者: Nasukawa, Tadahiro ; Matsui, Hidehito ; Ogata, Masaya ; Kato, Shin-ichiro ; Uchiyama, Jumpei ; Higashide, Masato ; Hanaki, Hideaki ; Takemura-Uchiyama, Iyo ; Murakami, Hironobu

Endolysin is a bacteriophage-derived enzyme that degrades the peptidoglycan in the cell wall of host bacteria; it could be used as an antimicrobial agent for selectively eliminating specific bacterial genera/species. Group B Streptococcus (GBS) causes neonatal infection via vertical transmission; prenatal GBS screening test, in which enrichment culture is followed by bacterial identification, is used to detect the presence of GBS in pregnant women.

2019-09-01·Enzyme and Microbial Technology3区 · 工程技术

Preparation and characterization of endolysin-containing liposomes and evaluation of their antimicrobial activities against gram-negative bacteria

3区 · 工程技术

Article

作者: Bai, Jaewoo ; Ryu, Sangryeol ; Chang, Pahn-Shick ; Yang, Eunhye

The overuse and misuse of antibiotics in treating bacterial infections cause the rapid emergence of drug-resistant bacteria, suggesting that the development of alternative strategies to control antibiotic-resistant bacteria is urgently needed. Endolysins are bacteriophage-encoded enzymes that can degrade peptidoglycan in bacterial cell walls, and they have great potential as alternative antimicrobial agents. However, exogenous application of recombinant endolysin is limited to Gram-positive bacteria because endolysins cannot penetrate the outer membrane of Gram-negative bacteria. Here, a liposome-mediated endolysin encapsulation system was developed, and its ability to penetrate the outer membrane of Gram-negative bacteria was tested. The phage-derived endolysin BSP16Lys was isolated, characterized, and used for encapsulation into a cationic liposome comprised of dipalmitoylphosphatidylcholine (DPPC), cholesterol, and hexadecylamine. The BSP16Lys-encapsulated liposome had a high zeta potential value (over 30 mV) with an average diameter of 303 nm. The encapsulation efficiency of BSP16Lys into the liposome was 35.27%. Salmonella Typhimuriumand Escherichia coli cells treated with BSP16Lys-encapsulated liposomes showed 2.2-log CFU/mL and 1.6-log CFU/mL reductions in the viable cell numbers, respectively, without treatment of a membrane permeabilizer. These results showed potential for liposome-mediated delivery of endolysin for exogenous application against Gram-negative bacteria.

2017-03-31·Journal of Exercise Nutrition & Biochemistry

Effects of Different doses of Silk Peptide on Energy Metabolism During Exercise in Mice

Article

作者: Suh, Heajung ; Kim, Jisu ; Lee, Chi-Ho ; Kim, Bokyung ; Park, Jonghoon ; Lim, Kiwon

PURPOSEThis study was carried out to determine the optimal dose of silk peptide for enhancing fat metabolism during exercise.METHODSFifty male ICR mice were randomly divided into five groups: Sed, SP0, SP200, SP400, and SP800. All SP mice underwent training by running on a treadmill 5 times a week for 2 weeks (20 m/min, 8° slope, 50 min/day for the first week and 25 m/min, 8° slope, 50 min/day at about 70-75% of maximum oxygen uptake for the second week).RESULTSAfter the 2 weeks, fat oxidation was measured during a 1-h exercise at the training conditions of the second week and was found to be 1.02 ± 0.15, 1.04 ± 0.17, 0.98 ± 0.10, 1.14 ± 0.19, and 1.15 ± 0.07 g/kg/h for Sed, SP0, SP200, SP400, and SP800 groups, respectively. The SP800 group had significantly higher fat oxidation levels than the SP0 group did at 36, 40, and 56 min and the Sed group did at 2, 4, 6, 8, 12, 14, 16, 20, 40, 46, 50, 52, 56, and 60 min. However, there was no significant difference among the groups in carbohydrate oxidation during the 1-h exercise. SP doses of 200 mg/kg and 400 mg/kg did not show any effect on fat and carbohydrate oxidation.CONCLUSIONIn conclusion, 800 mg/kg of silk peptide is the optimal dose for enhancing fat metabolism during exercise. In addition, silk peptide treatment could reduce body weight by enhancing fat metabolism.

100 项与 SP.800 相关的药物交易

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