Aflatoxin B1 (AFB1) exhibits high toxicity and wide-ranging hazards, causing substantial economic losses to human society. The insect-based method, as an emerging biological detoxification technology, owes its core advantage to the efficient AFB1-degrading apacity of Tenebrio molitor. Currently, research on the mechanism underlying AFB1 degradation by T. molitor remains scarce and superficial globally. Thus, based on the efficient AFB1-transforming ability of T. molitor, this study uses isobaric tags for relative and absolute quantitation (iTRAQ) to identify the key proteins and metabolic pathways involved in AFB1 degradation by T. molitor. Proteins highly associated with AFB1 detoxification in T. molitor included: Phase I metabolism-related enzymes: Cytochrome P450 monooxygenases (P450s) such as P450 6k1, CYP4Q34, CYP4Q32, CYP6BK19, CYP6BQ36, P450 6a2, CYP9AD2, and CYP347A6; esterases (ESTs) and esterase family members (FE4, CXE3, CXE14); carbonyl reductases, dehydrogenases, and juvenile hormone epoxide hydrolases. Phase II metabolism-related enzymes: Glutathione S-transferases (GSTs) and UDP-glucuronosyltransferases (UGTs). Phase III metabolism-related proteins: ATP-binding cassette (ABC) transporter family members, including G20, A3, and MRP1BX1. Results from in vitro and in vivo experiments using enzyme inhibitors targeting P450s and GSTs demonstrated that P450s, GSTs, and ESTs are critical enzymes in the AFB1 detoxification process of T. molitor. Heterologous expression of CYP4C1 (a member of the P450 enzyme family) was performed in Pichia pastoris; microsomes extracted from the recombinant strain achieved an AFB1 degradation rate of 82 %. HPLC-MS/MS analysis identified the degradation product as Aflatoxin M1 (AFM1), whose toxicity is significantly reduced. This study provides a theoretical basis for using T. molitor as an animal bioreactor for the detoxification of mycotoxin-contaminated grains and the biotransformation of AFB1-polluted raw materials, offering a novel approach to address food and feed safety challenges in China.

2026-01-01·AGEING RESEARCH REVIEWS

Contradictory dual role of antinutrients in nutrient inhibition and anti-aging: A comprehensive review on health benefits, processing techniques, and dietary strategies

Review

作者: Karabulut, Gulsah ; Günal-Köroğlu, Deniz ; Capanoglu, Esra ; Yılmaz, Hilal ; Erskine, Ezgi

BACKGROUND:

Antinutritional factors (antinutrients) are bioactive compounds in plant-derived foods that can impair nutrient absorption and reduce the bioavailability of proteins, carbohydrates, and essential minerals. Major representatives include phytates, oxalates, lectins, tannins, saponins, and enzyme inhibitors, which are associated with adverse nutritional outcomes through interference with nutrient uptake.

SCOPE AND APPROACH:

Recent studies reveal that antinutrients also possess bioactive properties with potential benefits for aging and longevity. This review assesses their dual role, considering both their inhibitory effects on nutrient utilization and their emerging therapeutic potential, and discusses dietary strategies and processing techniques that minimize risks while enhancing health-promoting properties.

KEY FINDINGS AND CONCLUSIONS:

Aging is driven by oxidative stress, inflammation, mitochondrial dysfunction, and cellular senescence, all of which contribute to cancer, cardiovascular disease, and neurodegeneration. Antinutrients may counteract these processes via antioxidant, anti-inflammatory, and autophagy-modulating actions. For instance, phytates protect against oxidative damage, saponins support mitochondrial function, and processing methods such as fermentation, soaking, and germination can reduce harmful effects while preserving bioactivity. Understanding these interactions highlights opportunities to harness antinutrients as functional compounds to promote healthy aging and extend healthspan.

2025-12-01·ChemistryOpen

Impact of Seasonal Variation on Antioxidant, Enzyme Inhibitory, and Anti‐Inflammatory Potentials of Cistus creticus Leaf Extracts

Article

作者: Benmohamed, Mokhtar ; Harchaoui, Lilya ; Sawicka, Barbara ; Bendrihem, Afaf Khadra ; Zahnit, Wafa ; Khattabi, Latifa ; Ferhat, Mohamed Amine ; Messaoudi, Mohammed ; Guelifet, Khalil ; Atoki, Ayomide Victor ; Ferhat, Abderrazek ; Kherraz, Khaled

This study investigates the influence of seasonal variation onthe phytochemicalcomposition and biological activities of Cistus creticus leaf extracts collected during spring, summer, autumn, and winter. Extracts are analyzed for phenolic and flavonoid contents and evaluated for antioxidant, enzyme inhibitory, anti‐inflammatory, analgesic, antimicrobial, and photoprotective properties. Pronounced seasonal differences are observed. Spring and summer extracts, enriched in bioactive compounds, exhibit the strongest pharmacological potential, including notable antioxidant effects, potent enzyme inhibition, and high photoprotective capacity. The spring extract further demonstrates significant in vivo anti‐inflammatory and analgesic effects, while the winter extract displays superior in vitro anti‐inflammatory activity. These findings highlight the critical role of harvest season in modulating both phytochemical composition and bioefficacy. The superior performance of spring and summer extracts underscores the potential of C. creticus as a valuable natural source of antioxidants, enzyme inhibitors, and photoprotective agents. Overall, this work supports the strategic use of seasonal optimization to enhance the therapeutic and cosmeceutical applications of C. creticus .

100 项与 TNCE-3 相关的药物交易

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