nutrients are closely related to consumer health. In this study, the contents of 22 amino acids in four glutinous rice varieties were quantified by LC-MS-targeted metabolomics, and the relationships between nutritional components and metabolites were analysed. The results demonstrated that gingerglycolipid B and pantothenic acid were significantly negatively correlated with Ala. The Lys and Cys-Cys contents of ZN18 and YZN were significantly higher than those of YCN and ZN20. Citric acid was significantly positively correlated with Lys and Cys-Cys. The ascorbic acid content of YZN and ZN18 was significantly higher than that of YCN and ZN20. YZN had the highest Mn, Zn, Ca, and crude fat contents. Gingerglycolipid B and pantothenic acid were significantly negatively correlated with Mn and Zn. Gingerglycolipid B and pantothenic acid are closely related to other nutritional components, and they are important metabolites formed by the nutrient components of purple glutinous rice.