2002-03-22·Journal of Biological Chemistry2区 · 生物学
Antiplasmin activity of a peptide that binds to the receptor-binding site of angiogenin
2区 · 生物学
作者: Gho, Yong Song ; Yoon, Wan-Hee ; Chae, Chi-Bom
It has been suggested that angiogenin binds to an actin-like molecule present on the surface of endothelial cells. Actin inhibits plasmin activity, but the angiogenin-actin complex is not active. In this report, we found that plasmin inhibits the interaction between angiogenin and actin suggesting a possibility that both angiogenin and plasmin may bind to a similar site on actin. Here we report that chANG, an antiangiogenin peptide that binds to the actin-binding site of angiogenin, inhibits the proteolytic activity of plasmin without any apparent effect on the activities of plasminogen activators and matrix metalloproteases. Its antiplasmin activity is comparable with that of actin. chANG inhibits plasmin activity via its binding to plasmin kringle domains while scrambled chANG does not bind to plasmin. chANG also inhibits the invasion of angiogenin-secreting human fibrosarcoma and colorectal carcinoma cells without effecting migration. Furthermore, chANG blocks angiogenesis induced by fibrosarcoma cells and metastasis of colorectal carcinoma cells to the liver. Therefore, the 11-amino acid peptide chANG has both antiangiogenin and antiplasmin activity, and could be useful in the development of anticancer agents.
1989-09-01·Zhong xi yi jie he za zhi = Chinese journal of modern developments in traditional medicine
[Effect of chang chun dan on noradrenaline levels of the brain and plasma in aged rats].
作者: L Y Cheng
In this paper, the rats were randomly divided into three groups: (1) Young (Y); (2) Aged (A); (3) Aged rats treated with Chang Chun Dan (AT) for 3 months. The level of noradrenaline (NA) in brain and plasma of three group rats had been examined by radioenzymatic assay. The changes of fluorescent intensity of NA in locus coeruleus had been observed by histochemical method. The levels of NA in hypothalamus of three groups were 162 +/- 9.93, 209 +/- 13.2, 167 +/- 11.8 ng/g wet tissues and in plasma were 1.29 +/- 0.16, 0.84 +/- 0.08, 0.96 +/- 0.08 ng/ml respectively. The results showed that the NA level of hypothalamus in AT group was lower than that of A group (P less than 0.05), and the NA level in Y group was raised markedly comparing with A group (P less than 0.01). The plasma NA level in A group was significantly lower than that in Y group (P less than 0.05). The NA level in AT group was raised a little comparing with A group. But the fluorescent intensity of NA in locus coeruleus between AT and A group was no difference. The results suggested that the changes of neurotransmitter induced by Chang Chun Dan may be basis of delaying aging effect and improving symptom of Kidney deficiency induced by aging.