Effects of dietary nanoplastics exposure on muscle quality, immunity, antioxidative capacity and digestive gland function of abalone (Haliotis discus hannai)

Article

作者: Zhang, Jiping ; Liu, Jiahuan ; Ren, Hai ; Fu, Qiyuan ; Cheng, Sisi ; Mu, Ye

The increasing accumulation of nanoplastics (NPs) in marine environment poses significant ecological and economic risks, particularly for commercially important species such as abalone (Haliotis discus hannai). This study investigated the effects of dietary polystyrene NPs (0,1, 10 and 100 mg/kg) on abalone over a 21-day exposure period, and the groups were designated as control, NPs1, NPs10 and NPs100, respectively. Results demonstrated that NPs impaired antioxidant capacity, as evidenced by elevated malondialdehyde (MDA) levels in cell-free hemolymph (1.83-fold higher in the NPs100 group than in control), together with reduced superoxide dismutase activity, decreased total antioxidant capacity, and inhibition of the Keap1/Nrf2 pathway. Immune suppression was observed through decreased lysozyme and acid phosphatase activities. Muscle texture deterioration was observed (muscle hardness decreased to 0.42-fold of the control level in NPs100 group), which was associated with reduced protein content, inhibition of the mTOR pathway, and upregulation of proteolytic genes (capn1, capn2, ctsl and ctsb). In the digestive gland, NPs induced lipid accumulation (increased to 1.20-fold of the control level in the NPs100 group), accompanied by suppressed lipolysis-related genes (atgl, hsl, cpt-1 and acox) and enhanced lipogenesis-related genes (srebp-1c, acc and scd) expression. Additionally, NPs upregulated inflammation-related genes (myd88, nf-κb, tnf-α and il-17) and apoptosis-related proteins (BAX, CytC and cleaved caspase-3), along with histopathological changes and reduced digestive enzyme activities. These findings indicate that NPs impair abalone health and muscle quality through oxidative stress, metabolic disruption, apoptosis and protein turnover. Primary adverse effects were mainly observed at ≥10 mg/kg after 21 days, with the most pronounced responses at 100 mg/kg. The mTOR, NF-κB, and Bax/caspase-3 signaling pathways were the main targets of the biological effects induced by NPs.

2022-05-01·Advanced science (Weinheim, Baden-Wurttemberg, Germany)1区 · 材料科学

Designing Self‐Assembling Chimeric Peptide Nanoparticles with High Stability for Combating Piglet Bacterial Infections

1区 · 材料科学

ArticleOA

作者: Tang, Qi ; Tan, Peng ; Zhang, Yucheng ; Xu, Shenrui ; Ma, Xi ; Fu, Huiyang

Abstract:

As a novel type of antibiotic alternative, peptide‐based antibacterial drug shows potential application prospects attributable to their unique mechanism for lysing the membrane of pathogenic bacteria. However, peptide‐based antibacterial drugs suffer from a series of problems, most notably their immature stability, which seriously hinders their application. In this study, self‐assembling chimeric peptide nanoparticles (which offer excellent stability in the presence of proteases and salts) are constructed and applied to the treatment of bacterial infections. In vitro studies are used to demonstrate that peptide nanoparticles NPs1 and NPs2 offer broad‐spectrum antibacterial activity and desirable biocompatibility, and they retain their antibacterial ability in physiological salt environments. Peptide nanoparticles NPs1 and NPs2 can resist degradation under high concentrations of proteases. In vivo studies illustrate that the toxicity caused by peptide nanoparticles NPs1 and NPs2 is negligible, and these nanoparticles can alleviate systemic bacterial infections in mice and piglets. The membrane permeation mechanism and interference with the cell cycle differ from that of antibiotics and mean that the nanoparticles are at a lower risk of inducing drug resistance. Collectively, these advances may accelerate the development of peptide‐based antibacterial nanomaterials and can be applied to the construction of supramolecular nanomaterials.

2021-05-01·The Plant journal : for cell and molecular biology1区 · 生物学

The new kid on the block: a dominant‐negative mutation of phototropin1 enhances carotenoid content in tomato fruits

1区 · 生物学

Article

作者: Sharma, Sulabha ; Dindu, Alekhya ; Dhanya, Ajayakumar Jaya ; Tyagi, Kamal ; Nizampatnam, Narasimha Rao ; Thazath, Nikhil Padmanabhan ; Kilambi, Himabindu Vasuki ; Gupta, Neha ; Sharma, Kapil ; Kumar, Sumit ; Sreelakshmi, Yellamaraju ; Sharma, Rameshwar

SUMMARY:

Phototropins, the UVA‐blue light photoreceptors, endow plants to detect the direction of light and optimize photosynthesis by regulating positioning of chloroplasts and stomatal gas exchange. Little is known about their functions in other developmental responses. A tomato Non‐phototropic seedling1 (Nps1) mutant, bearing an Arg495His substitution in the vicinity of LOV2 domain in phototropin1, dominant‐negatively blocks phototropin1 responses. The fruits of Nps1 mutant were enriched in carotenoids, particularly lycopene, compared with its parent, Ailsa Craig. On the contrary, CRISPR/CAS9‐edited loss of function phototropin1 mutants displayed subdued carotenoids compared with the parent. The enrichment of carotenoids in Nps1 fruits is genetically linked with the mutation and exerted in a dominant‐negative fashion. Nps1 also altered volatile profiles with high levels of lycopene‐derived 6‐methyl 5‐hepten2‐one. The transcript levels of several MEP and carotenogenesis pathway genes were upregulated in Nps1. Nps1 fruits showed altered hormonal profiles with subdued ethylene emission and reduced respiration. Proteome profiles showed a causal link between higher carotenogenesis and increased levels of protein protection machinery, which may stabilize proteins contributing to MEP and carotenogenesis pathways. The enhancement of carotenoid content by Nps1 in a dominant‐negative fashion offers a potential tool for high lycopene‐bearing hybrid tomatoes.

100 项与 NPS-1 相关的药物交易

登录后查看更多信息