There have been several studies regarding lichen-associated bacteria obtained from diverse environments. Our screening process identified 49 bacterial species in two lichens from the Himalayas: 17 species of Actinobacteria, 19 species of Firmicutes, and 13 species of Proteobacteria. We discovered five types of strong antimicrobial agent-producing bacteria. Although some strains exhibited weak antimicrobial activity, NP088, NP131, NP132, NP134, and NP160 exhibited strong antimicrobial activity against all multidrug-resistant strains. Polyketide synthase (PKS) fingerprinting revealed results for 69 of 148 strains; these had similar genes, such as fatty acid-related PKS, adenylation domain genes, PfaA, and PksD. Although the association between antimicrobial activity and the PKS fingerprinting results is poorly resolved, NP160 had six types of PKS fingerprinting genes, as well as strong antimicrobial activity. Therefore, we sequenced the draft genome of strain NP160, and predicted its secondary metabolism using antiSMASH version 4.2. NP160 had 46 clusters and was predicted to produce similar secondary metabolites with similarities of 5-100%. Although NP160 had 100% similarity with the alkylresorcinol biosynthetic gene cluster, our results showed low similarity with existing members of this biosynthetic gene cluster, and most have not yet been revealed. In conclusion, we expect that lichen-associated bacteria from the Himalayas can produce new secondary metabolites, and we found several secondary metabolite-related biosynthetic gene clusters to support this hypothesis.

1996-07-01·Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism

Lipid composition of the phosphatidylcholine-producing bacterium Hyphomicrobium vulgare NP-160

Article

作者: Denis I. Nikitin ; Stanislav G. Batrakov

The extractable lipids of the PC-producing, methylotrophic, budding bacterium Hyphomicrobium vulgare NP-160 grown in a mineral-salts medium containing methanol as the carbon source, were studied by chromatographic and spectrometric methods. They were found to be comprised of PC (35% of the total lipids), PDME (33%), PMME (1%), PE (9%), PG (10%), DPG (6%), and a non-phosphorus, ornithine-containing lipoamino acid, OL (6%). No low-polarity lipids, such as fatty acid esters of glycerol or of other alcohols, were detected. The sole fatty-acyl constituents of PDME and PMME were cis-octadec-11-enoic (cis-vaccenic) acid residues, whereas the other phospholipids contained, in addition, 1 to 5 mol % of MOA (lactobacillic acid) residues located predominantly at the sn-1 position of their glycerol residues. OL consisted of two molecular species, 2-N-[3'-(cis-octadec-11"-enoyloxy)octadecanoyl]-L- ornithine and 2-N-[3'-(cis-11", 12"-methyleneoctadecanoyloxy)octadecanoyl]-L-ornithine in the molar ratio 94:6. When the culture medium was devoid of phosphate, a threefold increase in OL together with a three-fold decrease in PE were observed, no significant changes in proportions of the remaining lipids occurring. The most striking feature of the lipid composition in this case was the presence of considerable amounts of fatty acid methyl esters, mainly methyl cis-vaccenate, along with minute amounts of wax esters.

1975-01-01·Genetika

Tsitogeneticheskiĭ analiz deĭstviia novykh bifunktsional'nykh alkiliruiushchikh soedineniĭ

Article

作者: Agabeili, R A

The mutagenic activity of nitrogen mustard derivatives of aminovinylketones (NP-130, NP-161, NP-163), aminovinylphosphoric acid (RVS-445, RVS-446, RVS-448, RVS-398) and ethylene imine derivatives of ethane sulphonic acid (AR, RJ) was established for the first time in the experiments with Allium fistulosum L. The mutagenic activity of aminovinylketones (NP-130, NP-160) was established for the first time in the experiments with Crepis capillaris (L.) Wallr. The effect of the compounds studied on the seedlings characterized by the high level of natural mutability of chromosomes did not alter the spectrum of structural chromosome mutations. As it is shown by the results of the analysis, the compounds studied exert their action mainly at the S stage of the cell cycle. However, the initiation of potential changes at the G1 stage that are realized into actual breaks at the S stage is not excluded. In some experiments in which high concentrations of mutagens were used no delayed effect was observed which might be explained by the action of mutagens on cells at the G2 stage or at a still later stage.

100 项与 NP-160 相关的药物交易

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