One-pot green synthesis of ZIF-8/IgG composite for the precise orientation and protection of antibody and its application in purification and detection of aflatoxins in peanut oil

Article

作者: Chen, Jinnan ; Shen, Wenjie ; Xuan, Zhihong ; Wang, Songxue ; Li, Li ; Ye, Jin ; Ni, Baoxia ; Lian, Ziye ; Liu, Hongmei ; Guo, Baoyuan

This study presents a novel approach toward the one-pot green synthesis of ZIF-8/IgG composite, focusing on its precise orientation and protection of the anti-aflatoxins antibody. The antibody orientation is achieved through the specific binding of IgG to the Fc region of the antibody, while the antibody protection is accomplished by the structural change restriction of ZIF-8 framework to the antibody. Consequently, the antibody exhibits enhanced target capability and significantly improved tolerance to organic solvents. The ZIF-8/IgG/anti-AFT was employed for the purification and detection of AFTs by coupling with UPLC. Under optimized conditions, the recoveries of spiked AFTs in peanut oils are between 86.1% and 106.4%, with relative standard deviations (RSDs) ranging from 0.8% to 8.8%. The linearity range is 0.5-20.0 ng for AFB₁ and AFG₁, 0.125-5.0 ng for AFB₂ and AFG₂, the limit of detection is 0.1 ng for AFB₁ and AFG₁, 0.03 ng for AFB₂ and AFG₂.

2024-07-01·Food Chemistry: Molecular Sciences

Morphed aflaxotin concentration produced by Aspergillus flavus strain VKMN22 on maize grains inoculated on agar culture

Article

作者: Okechukwu, Viola O ; Mamo, Messai A ; Njobeh, Patrick B ; Kappo, Abidemi P

This study identified and monitored the levels of aflatoxins (B₁ and B₂) produced by Aspergillus flavus isolate VKMN22 (OP355447) in maize samples sourced from a local shop in Johannesburg, South Africa. Maize samples underwent controlled incubation after initial rinsing, and isolates were identified through morphological and molecular methods. In another experiment, autoclaved maize grains were intentionally re-inoculated with the identified fungal isolate using spore suspension (106 spore/mL), after which 1 g of the contaminated maize sample was inoculated on PDA media and cultured for seven days. The aflatoxin concentrations in the A. flavus contaminated maize inoculated on culture media was monitored over seven weeks and then measured using liquid chromatography-mass spectroscopy (LC-MS). Results confirmed the successful isolation of A. flavus strain VKMN22 with accession number OP355447, which consistently produced higher levels of AFB₁ compared to AFB₂. AF concentrations increased from week one to five, then declined in week six and seven. AFB₁ levels ranged from 594.3 to 9295.33 µg/kg (week 1-5) and then reduced from 5719.67 to 2005 µg/kg in week six and seven), while AFB₂ levels ranged from 4.92 to 901.67 µg/kg (weeks 1-5) and then degraded to 184 µg/kg in week six then 55.33 µg/kg (weeks 6-7). Levene's tests confirmed significantly higher mean concentrations of AFB₁ compared to AFB₂ (p ≤ 0.005). The study emphasizes the importance of consistent biomonitoring for a dynamic understanding of AF contamination, informing accurate prevention and control strategies in agricultural commodities thereby safeguarding food safety.

2024-06-02·Food Additives & Contaminants: Part A

Exposure assessment and risk characterisation of aflatoxins in randomly collected rice samples from local markets of Hyderabad, India

Article

作者: Sagubandi, Yamuna ; Sinha, Sukesh Narayan ; Mungamuri, Sathish Kumar ; Gorain, Sumitra ; Validandi, Vakdevi ; Kurella, Srinivasu

Aflatoxin (AF) poisoning of staple foods, such as rice, is caused by fungal contamination by Aspergillus species. These AFs are genotoxic, carcinogenic and suppress the immune system. Hence, the present study was conducted to elucidate the prevalence of AF contamination in rice samples collected from local markets of Hyderabad, Telangana, India. The rice samples collected were analysed for AF by using HPLC-fluorescence detection (HPLC-FLD). Based on AF contamination levels and dietary intake of rice, the health risk was assessed by the margin of exposure (MOE) and liver cancer risk in adults, adolescence and children. The percentage detected contamination with AFB₁ and AFB₂ of rice samples was 54% and 34%, with the concentration ranging between 0-20.35 µg/kg and 0-1.54 µg/kg, respectively. Three rice samples exceeded the Food Safety and Standards Authority of India (FSSAI) total AF acceptable limit of 15 µg/kg. The average MOE values were 53.73, 50.58 and 35.69 (all <10,000) for adults, adolescence and children, respectively. The average liver cancer risk associated with rice consumption in the population of Hyderabad was found to be 0.27, 0.28 and 0.40 hepatocellular carcinoma (HCC) cases/year/100,000 individuals in adults, adolescence and children, respectively. This study revealed an adverse health risk to population of Hyderabad due to consumption of AF contaminated rice.

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