The shift towards eco-friendly solvents has driven interest in natural deep eutectic solvents (NADES) for advancing natural product research.Wedelia chinensis, celebrated in traditional medicine, is a non-conventional edible plant in India.Its therapeutic potential, particularly in diabetes management, positions it as a scientifically promising candidate for modern nutritional and pharmacol. research.However, the bioactive compounds and mechanisms underlying their anti-diabetic properties remain unclear.This study investigates the anti-diabetic potential of W. chinensis leaf extracts, prepared with both conventional solvents and eco-friendly natural deep eutectic solvents (NADES), to uncover novel therapeutic pathways for diabetes management.The bioactivity evaluations included assessments of total phenolic content (TPC) and total flavonoid content (TFC), antioxidant capacity, α-amylase/α-glucosidase inhibition, and anti-inflammatory activity.Docking studies investigated key bioactives against α-amylase and α-glucosidase, while network pharmacol. elucidated the mechanisms of action in diabetes management.A total of 81 secondary metabolites were identified.The Et acetate extract demonstrated the highest TPC (536.2 ± 3.7 mg GAE/g) and TFC (728.4 ± 12.4 mg RUE/g), along with superior antioxidant, enzyme inhibition, and anti-inflammatory activities.Among the NADES, NADES-4 exhibited the highest TPC (389.1 ± 4.0 mg GAE/g) and TFC (303.7 ± 24.9 mg RUE/g).Glycyrrhizin emerged as the top-performing compound in docking studies.The identified anti-diabetic mechanisms involved pathways such as linoleic acid and pyruvate metabolismThis interdisciplinary study highlights the potential of W. chinensis extracts as effective anti-diabetic agents, paving the way for the development of herbal medicines, functional foods, nutraceuticals, and innovative dietary interventions.

2025-04-01·PLANT PHYSIOLOGY AND BIOCHEMISTRY

The protective roles of boron against copper excess in citrus roots: Insights from physiology, transcriptome, and metabolome

Article

作者: Chen, Li-Song ; Lu, Fei ; Huang, Wei-Lin ; Huang, Zeng-Rong ; Chen, Xu-Feng ; Huang, Wei-Tao ; Shen, Qian ; Lai, Ning-Wei ; Yang, Lin-Tong

Boron (B) deficiency and copper (Cu) excess are common problems in citrus orchard soils. Citrus sinensis seedlings were exposed to 25 (B25) or 2.5 (B2.5) μM H₃BO₃ and 0.5 (Cu0.5) or 350 (Cu350) μM CuCl₃ for 24 weeks. Cu350 upregulated 2210 (1012) genes and 482 (341) metabolites and downregulated 3201 (695) genes and 175 (43) metabolites in roots at B2.5 (B25). Further analysis showed that the B-mediated mitigation of Cu toxicity in roots involved the coordination of the following aspects: (a) enhancing the ability to maintain cell wall and plasma membrane stability and function; (b) lowering the impairment of Cu350 to primary and secondary metabolisms and enhancing their adaptability to Cu350; and (c) alleviating Cu350-induced oxidative stress via the coordination of reactive oxygen species (ROS) and methylglyoxal detoxification systems. Cu350 upregulated the abundances of some saccharides, amino acids and derivatives, phospholipids, secondary metabolites, and vitamins, and the expression of several ROS detoxification-related genes in roots of B2.5-treated seedlings (RB2.5), but these adaptive responses did not prevent RB2.5 from Cu-toxicity (oxidative damage). The study identified some genes, metabolites, and metabolic processes/pathways possibly involved in root Cu tolerance. Additionally, the responses of gene expression and metabolite profiling to Cu-B treatments differed between leaves and roots. Therefore, this study provided novel information for B to reduce Cu toxicity in roots and might contribute to the development of soil amendments targeting Cu excess in citrus and other crops.

2024-12-01·Progress in Neuro-Psychopharmacology

Causal relationship between genetically determined plasma metabolites and stroke: A two sample Mendelian randomization study

Article

作者: Chen, Siqi ; Zhang, Enhao ; Huang, Yi ; Han, Liyuan

INTRODUCTION:

This study investigates the causal relationship between plasma metabolites and stroke.

METHOD:

The primary analytical approach employed was the inverse variance weighted (IVW) method, complemented by the weighted median (WM) and MR Egger methods for Additionally, validation of the identified plasma metabolites was performed using the Steiger test and LD linkage disequilibrium score. Furthermore, the main results were confirmed through data from the UK Biobank.

RESULT:

The IVW analysis revealed the most notable negative association found in X-17335 levels (OR [95 % CI]: 0.82 [0.72, 0.94]). On the other hand, the strongest positive effect was seen in the 5'-homophase (AMP) to phase ratio (OR [95 % CI]: 1.17 [1.03, 1.32]). Moving on to the validation dataset, the most significant positive effect was observed in the 13 HODE+9-HODE levels (OR [95 % CI]: 0.996 [0.993, 0.999]), whereas the most significant negative effect was seen in the Dihydroxide levels (OR [95 % CI]: 1.004 [1.00, 1.007]). Notably, Alpha ketoglutarate levels exhibited strong causal effects in both datasets (OR 0.908 [0.841, 0.981], p = 0.0144). Enrichment analysis highlighted the association of Alpha ketoglutarate levels with five plasma metabolites in metabolic pathways relevant to stroke, specifically Arginine biosynthesis, Butanoate metabolism, Citrate cycle (TCA cycle), Alanine, aspartate, and glutamate metabolism, and Lipid acid metabolism, all linked to oxoglutaric acid.

CONCLUSION:

The discovery dataset showed the most significant positive effect of the 5'-homophase (AMP) to phase ratio, while the validation dataset revealed the most significant positive effect of the 13 HODE+9-HODE levels. Additionally, alpha ketoglutarate may offer a potential protective effect on stroke by influencing five metabolic pathways that intersect with Oxoglutaric acid during the progression of the condition.

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适应症	最高研发状态	国家/地区	公司	日期
急性髓性白血病	临床前	德国	Johann Wolfgang Goethe-Universität Frankfurt am Main	2024-06-25
神经痛	临床前	德国	Johann Wolfgang Goethe-Universität Frankfurt am Main	2024-06-25