Activation of murine thymocyte subpopulations by the oxidizing mitogens: Evidence for a sialylated, mitogen-reactive site on both PNA-positive and PNA-negative cells

3区 · 医学

Article

作者: Kurt H. Stenzel ; Abraham Novogrodsky ; Blanka Shanitzki ; Amiran Ravid

The mitogenic effect of galactose oxidase (GO), neuraminidase/galactose oxidase (NAGO), IO₄^-, and phytohemagglutinin (PHA) for peanut agglutinin (PNA)-binding and -nonbinding cortical thymocytes was studied.PNA-neg. thymocytes responded to GO only after treatment with neuraminidase.This occurred without comitogens and upon addition of TPA, interleukin 1 (IL)-1 or -2.IO₄^- was a weak mitogen for PNA-neg. cells.Its mitogenic effect was enhanced by IL-1 or IL-2.PNA-pos. cells, provided with IL-2, responded to GO only after neuraminidase treatment.IO₄^- was also mitogenic in this subpopulation.PNA-pos. cells did not respond to GO, NAGO, IO₄^-, or PHA in the absence of IL-2, even when TPA or IL-1 was provided.Thus, the mitogenic sites for PNA-pos. cells, similar to those of PNA-neg. cells, are sialylated.

1985-06-01·Journal of immunology (Baltimore, Md. : 1950)

Human B lymphoblastoid cell lines provide an interleukin 1-like signal for mitogen-treated T lymphocytes via direct cell contact.

Article

作者: Suthanthiran, M ; Lipkowitz, S ; Stenzel, K H ; Novogrodsky, A ; Arnold, A

The B lymphoblastoid cell lines (B-LCL) 8392, SB, 1788, and Daudi provide accessory cell activity for mitogen-treated T cells, whereas the T lines MOLT-4, 8402, CEM, and HSB do not provide this function. Direct cell contact is required for the accessory cell activity, and active lymphocyte growth factors could not be detected in the supernatants of the B-LCL. The B-LCL also present alloantigens to responding T cells, and this response is independent of additional accessory cells. The target for the B-LCL is the responding T cell itself, rather than a minor contaminating population of endogenous accessory cells. This conclusion is based on the finding that, under culture conditions in which T cells do not proliferate in response to PHA, accessory cell activity of the B-LCL is maintained. Paraformaldehyde- or glutaraldehyde-treated B-LCL retain their accessory cell activity at levels of these agents that completely eliminate metabolic activity of the B-LCL, as determined by incorporation of leucine, thymidine, and uridine into macromolecules. This treatment eliminates alloantigen presentation by the B-LCL. T cells treated with IO-4 or with monoclonal anti-T3 antibodies fail to respond to highly purified IL 1, and respond minimally to supra-optimal concentrations of IL 2. Nevertheless, these cells respond maximally to the accessory cell activity of the B-LCL. The IO-4 treated cells or cells exposed to anti-T3 also proliferate in response to TPA. Together, our data suggest that the B-LCL provide an IL 1-like signal for mitogen-treated T cells via direct cell contact, in the absence of detectable soluble IL 1.

1984-04-01·Cellular Immunology3区 · 医学

Induction and amplification of T-lymphocyte proliferative responses to periodate and soybean aggtutinin by human adult vascular endothelial cells

3区 · 医学

Article

作者: Jhomas J. Parsons ; Louis Ercolani ; Glenna L. Fry ; Dai D. Nghiem ; John C. Hoak

Human mononuclear phagocyte (M phi) populations were compared to adult human endothelial cells (HEC) for their respective abilities to influence the proliferative responses of purified human T lymphocytes to the mitogenic agents Na-m-periodate (IO-4), soybean agglutinin (SBA), or allogeneic cells. HEC and M phi were both capable of inducing proliferative responses of allogeneic T lymphocytes in mixed-lymphocyte culture. Under low cell density culture conditions, purified T-lymphocyte proliferative responses to IO-4 or SBA could be restored by addition of syngeneic M phi or HEC. At higher cell density culture conditions, proliferation of T cells to IO-4 could be amplified more by HEC than M phi. T-lymphocyte proliferative responses to SBA were amplified by addition of HEC but were suppressed by addition of M phi. These findings indicate that human adult HEC are unique and potent accessory cells for T lymphocytes. Furthermore, these findings demonstrate that accessory cell functions of HEC can be discriminated from those of M phi.

100 项与 ios H4 相关的药物交易

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