Article
作者: Kamen, Amine ; Laliberté-Gagné, Marie-Ève ; Fillion, Maude ; Savard, Pierre ; Bolduc, Marilène ; Gilbert, Mégan ; Rabezanahary, Henintsoa ; Xu, Xingge ; Olivera-Ugarte, Santa-Mariela ; Blanchette, Léa-Jeanne ; Scarrone, Martina ; Farnòs, Omar ; Dubuc, Isabelle ; Couture, Christian ; Garneau, Caroline ; Baz, Mariana ; Leclerc, Denis ; Flamand, Louis
A vaccine candidate to SARS-CoV-2 was constructed by coupling the viral receptor binding domain (RBD) to the surface of the papaya mosaic virus (PapMV) nanoparticle (nano) to generate the RBD-PapMV vaccine. Immunization of mice with the coupled RBD-PapMV vaccine enhanced the antibody titers and the T-cell mediated immune response directed to the RBD antigen as compared to immunization with the non-coupled vaccine formulation (RBD + PapMV nano). Anti-RBD antibodies, generated in vaccinated animals, neutralized SARS-CoV-2 infection in vitro against the ancestral, Delta and the Omicron variants. At last, immunization of mice susceptible to the infection by SARS-CoV-2 (K18-hACE2 transgenic mice) with the RBD-PapMV vaccine induced protection to the ancestral SARS-CoV-2 infectious challenge. The induction of the broad neutralization against SARS-CoV-2 variants induced by the RBD-PapMV vaccine demonstrate the potential of the PapMV vaccine platform in the development of efficient vaccines against viral respiratory infections.