Nooglutyl

A new synthesis of N-(5-hydroxynicotinoyl)-L-glutamic acid via a 5-hydroxynicotinic acid imidazolide intermediate has been developed.Its calcium salt (Ampasse) has been synthesized and its structure was studied by X-ray diffraction anal.The reaction conditions for all stages of the process have been optimized and a method for the purification of the substance has been improved.

Optimal conditions are found for the simultaneous determination of tranexamic acid, ampasse, and ethylmethylhydroxypyridine succinate by reversed-phase HPLC.As is shown, under these conditions the reversed-phase mechanism of retention prevails and the analytes occur mainly in a specific ionic state with an optimal retention time.The possibility of using chromatog. columns from various manufacturers is estimatedThe method was used to analyze title materials and impurities in drug formulations.