Coral holobionts constitute the foundational organisms of coral reef ecosystems. As an emerging pollutant, the projected accumulated levels of microplastics (MPs) are expected to continue increasing. Meanwhile, due to their properties, MPs can absorb multiple other marine pollutants, such as antibiotics (ATs). However, the co-toxicity mechanism of MPs and ATs to coral holobionts remains to be explored. Here, using Zoanthus sociatus as a model organism, we investigate the individual and combined toxicity of MPs and tetracycline (TC) at environment-related concentrations to coral holobionts. Microbiomics indicate that MPs and TC increase coral holobionts bacterial species richness while concurrently reducing the microbial community structure stability. The key metabolites and enzyme activity results demonstrated that the impacts of MPs and TC on corals encompassed antioxidant capacity, detoxification capability, immune function, and lipid metabolism. Transcriptomics shows that MPs and TC disrupt coral-algae relationships mainly through host nutrition limitation and inhibition of symbiotic algae carbon/nitrogen metabolism, respectively. A synergistic effect between MPs and TC has also been observed. In contrast, coral holobionts have shown adaptability through activating coral-symbiodiniaceae-bacteria interactions, mainly including: 1) enhancing the abundance of BMCs (beneficial microorganisms for corals); 2) enhancing host lipid accumulation; 3) immunoregulation; 4) symbiotic regulation. Overall, our findings provide new insights into the co-toxicity of MPs and TC, and highlight those MPs and TC at current environment concentration and predicted for most oceans in the coming decades, can ultimately cause coral bleaching.

2025-06-01·Journal of Hazardous Materials

Integration of machine learning and meta-analysis reveals the behaviors and mechanisms of antibiotic adsorption on microplastics

Article

作者: Xu, Jinling ; Liu, Enfeng ; Liu, Xiang ; Zhao, Shuling ; Chen, Hongfeng ; Ma, Xinru ; Wu, Ruoying ; Wei, Peng ; Bi, Shuangshuang

Microplastics (MPs) can adsorb antibiotics (ATs) to cause combined pollution in the environment. Research on this topic has been limited to specific types of MPs and ATs, resulting in inconsistent findings, particularly for the influencing factors and adsorption mechanisms. Therefore, this study combined meta-analysis and machine learning to analyze a dataset comprising 6805 records from 123 references. The results indicated that polyamide has the highest adsorption capacity for ATs, which is primarily attributed to the formation of hydrogen bonds by its N-H groups, and MPs exhibited the strongest affinity for chlortetracycline because the CO and -Cl groups in chlortetracycline form hydrogen and halogen bonds with MPs. Moreover, the particle size, MP and AT concentrations, and pH were key factors affecting the adsorption process with notable interaction effects. Hydrogen bonding and electrostatic interaction were commonly involved in the adsorption of ATs onto MPs. Finally, an interactive graphical user interface was deployed to predict the adsorption amount, affinity constant, and maximum adsorption capacity of MPs for ATs, with results aligning well with the latest published data. This study provides crucial insights into the behavior of MPs carrying ATs, thereby facilitating accurate assessment of the combined environmental risks of them.

2025-05-01·Forensic Science International

Development, validation and applications of a GC/MS method for the simultaneous determination of 9 amphetamines and 12 NPS analogues in blood and urine

Article

作者: Nikolaou, Panagiota ; Athanaselis, Sotirios ; Bouzoukas, Christoforos ; Dona, Artemisia ; Papoutsis, Ioannis ; Spiliopoulou, Chara

Amphetamine-type stimulants (ATS), synthetic cathinones (SCs) and phenethylamines (PEAs) pose a challenge to toxicology laboratories. Their extensive use and misuse has led to an increase of intoxications and fatal incidents worldwide. So, the need for new analytical methods for their determination in biological samples is crucial for all toxicology laboratories for a better investigation of these cases. The aim of this study was the development and validation of an analytical method for the determination of 9 ATS, 7 SCs and 5 PEAs in whole blood and urine using gas chromatography coupled with mass spectrometry. The samples were pretreated by solid-phase extraction and derivatized with pentafluoropropionic anhydride. Chromatographic separation of the 21 analytes was achieved in less than 11 min. The method was validated according to international guidelines for selectivity, specificity, linearity, limits of detection (LODs) and quantification (LOQs), precision, accuracy and recovery of the method. The LODs of the analytes ranged from 0.70 to 7.0 ng/mL and the values of the LOQs ranged from 2.0 to 20 ng/mL. The linearity of the 21 analytes, according to the group of substances, were 2.0 ng/mL - 0.20 μg/mL, 5.0 ng/mL - 0.50 μg/mL, 10 ng/mL - 0.50 μg/mL and 20 ng/mL - 0.50 μg/mL with R² values > 0.99. Extraction recoveries were > 80 % for all analytes. Intra and inter day accuracy and precision of the method were within accepted limits. The developed method was applied to post-mortem blood and urine samples of 46 forensic cases sent for toxicological investigation to the Department of Forensic Medicine and Toxicology, School of Medicine, National and Kapodistrian University of Athens. The results of the analyses revealed the presence of methamphetamine, amphetamine, MDMA, MDA, pseudoephedrine, phenylpropanolamine or MBDB in 14 cases.

100 项与 Antistasin(Merck Sharp & Dohme Corp.) 相关的药物交易

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