AbstractOccasionally, tissue samples cannot be processed completely and are stored under varying conditions for extended periods. This is particularly beneficial in interinstitutional studies where a given research setting may lack the expertise or infrastructure for sample processing, imaging and data analysis. Currently, there is limited literature available on the controlled storage of biological tissues in primary fixatives for fluorescence and electron microscopy. In this contribution, we mimicked various tissue storage scenarios by taking different fixation conditions, storage temperatures and storage durations into account. Rat liver tissue was used for its well-known diversity of cellular ultrastructure and microscopy analysis. Fluorescent labelling of actin, DNA and lipids were employed in conjunction with high-resolution electron microscopy imaging. Herein, we tested three different fixative solutions (1.5% glutaraldehyde, 0.4% glutaraldehyde and 4% formaldehyde and 4% formaldehyde) and stored samples for 1–28 days at room temperature and refrigerator temperature. We found that liver tissue can be stored for up to 2 weeks in a 0.4% glutaraldehyde + 4% formaldehyde fixative solution, while still enabling reliable fluorescent labelling and ultrastructural studies. Ultrastructural integrity was eminent up to 1 month using either glutaraldehyde or formaldehyde fixation protocols. When liver tissue is fixed with a mixture of 0.4% glutaraldehyde and 4% formaldehyde and stored at 4 °C, it retains its capacity for electron microscopy analysis for several years, but loses its capacity for reliable fluorescent labelling studies. In conclusion, we demonstrated that liver tissue can be stored for extended periods enabling profound structure–function analysis across length scales.

2025-07-01·Separation and Purification Technology

Highly efficient and selective adsorption of Hg(II) from water by N, S-functionalized fly ash-based tobermorite

作者: Liu, Yiwen ; Chen, Jianxin ; Jiang, Delu ; Wang, Zehua ; Zhou, Yongwei

Fly ash-based tobermorite (TFA) synthesized by hydrothermal reaction was successively modified by 3-aminopropyltriethoxysilane (APTES) and amidinothiourea (AT) to produce a novel N, S-functionalized adsorbent (TFA-N2S6) for selective adsorption of Hg(II) from water.TFA-N2S6 was characterized using FTIR, XRD, XPS, SEM-EDX, and BET.The results indicated that TFA-N2S6 was rich in NH₂ and C=S, and exhibited a high adsorption capacity of 276.16 mg/g for Hg(II), exceeding that of many other adsorbents.TFA-N2S6 achieved a 96 % adsorption efficiency for Hg(II) (100 mg/L) at pH 3-6.The adsorption of Hg(II) by TFA-N2S6 conformed to the Langmuir model and the pseudo-second-order model as a monolayer chemisorption process with spontaneous heat absorption.The impact of anions, cations, and humic acids on Hg(II) adsorption by TFA-N2S6 was studied, revealing a selective adsorption efficiency consistently above 80 %.The adsorption mechanism was mainly to form coordination complexes with Hg(II) in the form of S-Hg-N and S-Hg-S.Regeneration studies using thiourea solution as a regenerant showed good reusability of TFA-N2S6.

2025-07-01·Separation and Purification Technology

3D crosslinked chitosan for fluoride remediation in industrial wastewater: From structure to performance enhancement

作者: Wang, Qin ; Liu, Fengzhen ; Hao, Hongxun ; Li, Yan ; Zhou, Zijian ; Wang, Ting ; Wang, Na ; Huang, Xin

The development of novel adsorbent materials represents a promising approach for the remediation of fluorine pollution and the promotion of a sustainable water cycle.Nevertheless, the complex preparation processes, stringent application conditions, and limited reusability significantly impede the practical implementation of various adsorption materials.Herein, three-dimensional crosslinked chitosan (3D CS) was developed for treating wastewater containing high concentration of fluoride (hundreds and thousands of mg/L).FTIR, FE-SEM and other techniques were used to investigate several key process factors on the structure and performance of 3D CS and the optimal synthesis conditions, including chitosan hydrogel concentration (5%wt), the amount of crosslinking agent (2.5%volume/volume), acetic acid concentration (2%), drying method (353 K blast drying for 9 h), were determinedAfterwards, the optimized 3D CS demonstrates microporous structure and exceptional fluorine removal capabilities, characterized by a 6-fold enhancement in adsorption capacity (64.7 mg/g), a 5-fold improvement in recycling capacity (more than 17 times), as well as broad pH applicability range (3-12) and high salt tolerance (25000 mg/L NaCl and 5000 mg/L Na₂SO₄).Furthermore, its straightforward and low-carbon synthesis method, customizable macro shape, and ease of separation from wastewater endow the 3D CS with significant potential for practical applications.Ultimately, the adsorption mechanism was investigated and the results show that ion exchange, electrostatic attraction, and hydrogen bonding between 3D CS and HF together determine the good adsorption performance of F^-.

100 项与 Glutaral 相关的药物交易

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