Determination of 5-OH-DMT (bufotenine) in the Asian toad Duttyphrynus melanostictus, Hoplobatrachus tigerinus and Haemopis sanguisiga in Madagascar by UHPLC-MS/MS

Article

作者: Salomone, Alberto ; Bezandry, Brigitte Annie ; Ververi, Christina ; Rahantavololona, Vonimanitra Juliana Rasoma ; Nahavitatsara, Elisah Rasoanomenjanahary ; Giacoma, Cristina

5-hydroxy-N,N-dimethyltryptamine (5-OH-DMT), known as bufotenine, is proven to have psychoactive effects in high concentrations. Duttyphrynus melanostictus, which produces bufotenine, has migrated to the city of Toamasina in Madagascar, thus, the determination bufotenine's levels in the species' samples is necessary. This study aimed to quantify bufotenine in eggs, tadpoles and toad's skin samples of the Duttyphrynus melanostictus as well as in its predator Hoplobatrachus tigerinus and Haemopis sanguisiga. Two extraction protocols were applied depending on the sample type both based on liquid liquid extraction. Spiked calibration samples were prepared at six concentration levels (10-1000 ng/mL), and performance parameters were evaluated: linearity, accuracy (bias%), precision (CV%) and limit of detection. The proposed protocol is simple and quick, and the UHPLC-MS/MS system used exhibited adequate sensitivity. The target analyte was detected in all samples except for the eggs of Duttyphrynus melanostictus, with concentrations increasing in line with the animal's development.

2025-01-01·Food Chemistry

Targeted/untargeted metabolomics and antioxidant properties distinguish Citrus reticulata ‘Chachi’ from Citrus reticulata Blanco

Article

作者: Li, Xiang ; Wang, Xue ; Fu, Fuhua ; Su, Zhipeng ; Shan, Yang ; Chen, Jiaxu ; Pan, Zhaoping ; Li, Gaoyang

Dried citrus peel (DCP), also called "Chen Pi", has edible and medicinal value. However, the specific differences among various sources remain unknown. Herein, we collected six DCP species, namely, one Citrus reticulata 'Chachi' (CZG) and five Citrus reticulata Blanco (CRB). Targeted high-performance liquid chromatography and untargeted ultra-high-performance liquid chromatography-tandem mass spectrometry were employed to comprehensively compare the phenolic compounds and metabolites in DCP. Interestingly, 13 different phenolic compounds were noted in DCP. The total phenolic compound content in all CRB samples (58.86-127.65 mg/g) was higher than that of CZG (39.47 mg/g). Untargeted metabolomic revealed 1495 compounds, with 115 differentially expressed metabolites for CRBs and CZG, particularly flavonoids (38), terpenoids (15), and phenolic acids and derivatives (9). Lastly, antioxidant assays revealed that all CRB samples exhibited higher antioxidant activities compared with CZG. Therefore, our study results provide a theoretical basis for the high-value utilization of citrus peels and their metabolites.

2025-01-01·Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

Suppression of intestinal Ticam1 ameliorated MASH via Akkermansia muciniphila QAA37749.1 mediated betaine transformation

Article

作者: Guan, Lei ; He, Ruohang ; Seki, Ekihiro ; Yang, Bowen ; Li, Zhonglin ; Wang, Weijun ; Yuan, Ruiqing ; Chen, Yue ; Lin, Rong ; Yuan, Hang ; Hu, Lilin ; Zhang, Lei ; Pan, Xiaoli ; Chu, Huikuan ; Zhu, Qingjing ; Yang, Ling ; Cheng, Zilu ; Gao, Wenkang ; Liang, Minglu

BACKGROUND & AIMSGut inflammation caused by diets could damage the intestinal barrier, which increases the liver exposition to pathogenic substances. Toll-IL-1 receptor (TIR) domain-containing adaptor molecule-1 (Ticam1) is a key molecule in the Toll-like receptors (TLRs) pathway, which is important for the immune defense against pathogens such as bacteria or viruses. In this study, mouse intestinal epithelial cell (IEC) Ticam1 was knocked out to suppress the intestinal inflammation response in metabolic dysfunction-associated steatohepatitis (MASH) to investigate its influence on the development of MASH.METHODSThe IEC-specific Ticam1 knockout (Ticam1^ΔIEC) mice and the control (Ticam1^fl/fl) mice were fed with high-fat high-fructose diet (HFD) for 22 weeks to evaluate the gut alteration and the MASH-associated disorders. The intestinal secreted immunoglobulin A (sIgA) and IgA-secreting immune cells were detected. Shotgun metagenomic sequencing was used to find the gut microbiome shift in different groups. Liquid chromatography mass spectrometry was also performed to evaluate the change of serum metabolites caused by the gut microbiome alteration.RESULTSThe gut inflammation and gut barrier dysfunction were both alleviated in HFD-fed Ticam1^ΔIEC mice, which had improved MASH disorders compared with Ticam1^fl/fl. Additionally, HFD-fed Ticam1^ΔIEC mice had increased sIgA and intestinal IgA-secreting immune cells. It showed a significantly higher content of Akkermansia muciniphila. We proved that Akkermansia muciniphila encoded a protein named QAA37749.1 that could promote the conversion of choline to betaine, through which the development of MASH was inhibited in HFD-Ticam1^ΔIEC mice.CONCLUSIONDeletion of IEC Ticam1 alleviated MASH disorder and gut dysfunction in mice. It enhanced the level of intestinal sIgA and the growth of Akkermansia muciniphila, which supported the betaine transformation by QAA37749.1. Suppressing IEC Ticam1 might be a promising strategy for MASH disorder.

100 项与 Bufotenine 相关的药物交易

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