GE37468A is a new thiazolyl peptide antibiotic acting on Elongation Factor Tu, produced by fermentation of Streptomyces sp. ATCC 55365. The low (< 10 mg/liter) and poorly reproducible antibiotic productivity of the original soil isolate hampered the development of a scalable fermentation process. A tenfold increase of GE37468A productivity was achieved by selection of spontaneous morphochromatic variants, which appeared at high frequency in the wild type population. The higher producing ATCC 55365/O/5 colony was tyrosinase defective and blocked in the aerial mycelium differentiation (Amy-Mel- phenotype). The fermentation process of this stable and purified strain was rapidly optimized to achieve a further fourfold improvement in a fed-batch process.

1995-01-01·The Journal of Antibiotics4区 · 医学

Antibiotic GE37468 A: A Novel Inhibitor of Bacterial Protein Synthesis. II. Structure Elucidation.

4区 · 医学

Article

作者: Ferrari, P. ; Zerilli, L. F. ; Colombo, L. ; Stella, S. ; Selva, E.

GE37468 A is a novel antibiotic produced by Streptomyces sp. ATCC 55365. It has molecular mass 1309.48 and formula C59H52O12N14S5 and belongs to the thiazolyl peptide group of antibiotics. The structure was elucidated by 1H and 13C NMR and MS studies on intact molecule and its hydrolysis products. The antibiotic is a highly modified peptide containing a macrocycle and a side chain composed of a thiazole ring and two dehydroalanine units.

1995-01-01·The Journal of Antibiotics4区 · 医学

Antibiotic GE37468 A: A New Inhibitor of Bacterial Protein Synthesis. I. Isolation and Characterization.

4区 · 医学

Article

作者: Monnier, Francis Le ; Colombo, Luigi ; Selva, Enrico ; Montanini, Nicoletta ; Landini, Paolo ; Ferrari, Pietro ; Goldstein, Beth P. ; Denaro, Maurizio ; Stella, Sergio ; Ciciliato, Ismaela

GE37468 A is a new thiazolyl peptide antibiotic obtained by fermentation of Streptomyces sp. strain ATCC 55365. It inhibits bacterial protein synthesis by acting on elongation factor Tu and is structurally and functionally related to the GE2270 class of EF-Tu inhibitors. It is active in vitro against Gram-positive bacteria and Bacteroides fragilis, and protects mice against Staphylococcus aureus infection.

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