Naproxen Sodium/Metoclopramide Hydrochloride

A mechanically stable cellulose-based chromatography media was synthesized to permit inexpensive affinity purification of recombinant proteins containing the family 9 carbohydrate-binding module (CBM9) fused to either the N- or C-terminus of the target protein. A second-order response surface model was used to identify optimal concentrations of the primary reactants, epichlorohydrin and dimethyl sulfoxide (DMSO), required to cross-link the starting material, Perloza MT100, a compressible but inexpensive cellulose-based chromatography resin. This resulted in a mechanically stable cross-linked affinity chromatography media capable of operating at an order-of-magnitude higher linear velocity than permitted by unmodified MT100. Moments and Van Deemter analyses were used to show that rates of solute mass transfer within the column are largely unaffected by the cross-linking reaction, while the binding capacity decreased by 20% to 7.1 micromol of protein/g resin, a value superior to most commercial affinity chromatography media. In sharp contrast to MT100, the mechanical stability and purification performance of the cross-linked media are not diminished by scale-up or repeated column use.

In the present experiment male and female eelpout, Zoarces viviparus were exposed to different doses of the model androgen 17alpha-methyltestosterone (MT) and the effects on the plasma level of vitellogenin and the gonadosomatic index were investigated. In females exposed to different doses of MT (nominal concentrations 10, 25, 50, 100 and 500 ng/L) in the ambient seawater, the concentrations of the circulating yolk-precursor protein vitellogenin (vtg) were shown to decrease in all groups but only significantly in the MT-100 group when compared to controls. No significant effects could be observed on the GSI during early vitellogenesis (April/May). Males exposed to E2 under flow through conditions during 10 days showed an induced synthesis of vtg as depicted by a high level of circulating vtg. When the estrogen-exposed males were subsequently exposed to different doses of MT only, during another 10 days, the levels of vtg decreased significantly in three of the MT-treated groups when compared with the level of vtg in plasma of the estrogen-treated group after the first 10 days. The gonadosomatic index was observed to decrease by the estradiol-treatment but to increase in a dose-dependent manner in the MT-exposed groups, indicating that MT had the capacity to override the effect of the preceding exposure to estradiol-17beta.