作者: Buller, Christopher E. ; Henriques, Jose P. S. ; Goodhart, David ; Graham, John J. ; Dick, Alexander ; Vo, Minh N. ; Jolly, Sanjit S. ; Strauss, Bradley H. ; Schaempert, Erick ; Mancini, G. B. John ; Rinfret, Stéphane ; Weisz, Giora ; Teefy, Patrick ; Mansour, Samer ; Dzavik, Vladimir ; Elbarouni, Basem ; Wijeysundera, Harindra ; Bagai, Akshay

AbstractBackgroundChronic total occlusions (CTO) are common and are associated with lower percutaneous coronary intervention (PCI) success rates, often due to failure of antegrade guidewire crossing. Local, intralesional delivery of collagenase (MZ‐004) may facilitate guidewire crossing in CTO.AimsTo evaluate the effect of MZ‐004 in facilitating antegrade wire crossing in CTO angioplasty.MethodsA total of 76 patients undergoing CTO PCI were enrolled at 13 international sites: 38 in the randomized training stage (collagenase [MZ‐004] 900 or 1200 μg) and 38 in the placebo‐controlled stage (MZ‐004 900 or 1200 μg or placebo). Patients received the MZ‐004 or identical volume saline (placebo group) in a double‐blind design, injected via microcatheter directly into the proximal cap of the CTO. The following day patients underwent CTO PCI using antegrade wire techniques only.ResultsPatients were generally similar except for a trend for higher Japanese chronic total occlusion (J‐CTO) score in the MZ‐004 group (MZ‐004 J‐CTO score 1.9 vs. 1.4, p = 0.07). There was a numerical increase in the rates of guidewire crossing in the MZ‐004 groups compared to placebo (74% vs. 63%, p = 0.52). Guidewire crossing with a soft‐tip guidewire (≤1.5 g tip load) was significantly higher in the MZ‐004 groups (0% in placebo, 17% in 900 μg, and 29% in 1200 μg MZ‐004 group, p = 0.03). Rates of the major adverse cardiovascular event were similar between groups.ConclusionLocal delivery of MZ‐004 into coronary CTOs appears safe and may facilitate CTO crossing, particularly with softer tipped guidewires. These data support the development of a pivotal trial to further evaluate this agent.

2021-03-01·Experimental Eye Research3区 · 医学

Study on patterned photodynamic cross-linking for keratoconus

3区 · 医学

Article

作者: Huang, Yu ; Mi, Shengli ; Feng, Yun ; Chen, Yun ; Wei, Wei ; Ye, Cheng ; Hu, Yingbing

PURPOSEThis study examined the patterned treatment of corneal collagen cross-linking (CXL) for keratoconus to reduce the complications caused by ultraviolet (UV) irradiation. By modifying the method of UV irradiation during the cross-linking process, cross-linking with a special structure is achieved, and the cross-linking effect is analyzed and compared to that of traditional cross-linking. By constructing an animal model of keratoconus, the process and effect of corneal cross-linking can be investigated more fundamentally. These studies provide valuable references for future cross-linking precision improvement and specialization.METHODBy injecting exogenous collagenase into the corneal stroma of rabbits, the balance between collagenase and collagenase inhibitor in the corneal stroma was disrupted, the collagen fiber structure of the cornea was broken to simulate the pathogenesis of keratoconus, and an animal model of keratoconus was thus constructed. Two custom cross-linking patterns were designed with reference to the cable dome structure, and these two special patterns were irradiated and cross-linked by a DMD chip. The cross-linking effect was evaluated by optical coherence tomography (OCT), corneal topography and corneal biaxial tensile tests. The experimental rabbits were divided into four groups: group A, cross-linking of the bird's nest structure; group B, cross-linking of the honeycomb structure; group C, cross-linking of the traditional spot structure; and group D, normal (without modeling and cross-linking).RESULTFollowing collagenase treatment, the collagen fiber structure of the rabbit cornea was destroyed, the central thickness of the cornea was reduced, the mechanical properties of the cornea were weakened, and no keratitis, ulcers or haze occurred. After the three cross-linking treatments, the morphology of the cornea improved, the density of the stromal layer increased, and the mechanical properties were enhanced. For the improvement of keratoconus mechanical properties, the average relative difference (Δ) of the four outcome measures was 61.98% for bird's nest cross-linking versus keratoconus (Wilcoxon rank sum test, P = 0.024), 16.13% for honeycomb cross-linking versus keratoconus (Wilcoxon rank sum test, P = 0.025), and 21.07% for traditional spot cross-linking versus keratoconus (Wilcoxon rank sum test, P = 0.014). All these differences are statistically significant.CONCLUSIONAll three methods of cross-linking can improve the morphology and tissue structure of keratoconus and significantly improve the biomechanical properties of the cornea. Among them, the corneal cross-linking of the bird's nest structure attains the best biomechanical properties, followed by the corneal cross-linking of the traditional spot structure and of the honeycomb structure. This suggests that similar or better cross-linking effects can be achieved by designing custom structures with less UV exposure. This provides a direction for future research on better and more accurate pattern cross-linking treatments.

2018-10-01·Journal of Ethnopharmacology2区 · 医学

Anti-aging properties of Curcuma heyneana Valeton & Zipj: A scientific approach to its use in Javanese tradition

2区 · 医学

Article

作者: Maat, Suprapto ; Matsunami, Katsuyoshi ; Hestianah, Eka Pramyrtha ; Rullyansyah, Subhan ; Kurniawan, Kresma Oky ; Prijo, Tri Anggono ; Ekowati, Juni ; Widyowati, Retno ; Kusumawati, Idha

ETHNOPHARMACOLOGICAL RELEVANCETemu giring, the rhizome of Curcuma heyneana Valeton & Zipj (C. heyneana), is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory.AIM OF THE STUDYThe purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use.MATERIALS AND METHODSThe antioxidant activity was determined using the DPPH free radical method, and anti-aging activity was examined using in vitro assays such as tyrosinase inhibitor and collagenase inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) - densitometry.RESULTSIn all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase inhibitor and collagenase inhibitor activity greater than all C. heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma heyneana.CONCLUSIONSThe results show that C. heyneana contains antioxidant compounds and has potent anti-aging activity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical.

100 项与 Collagenase injection(Matrizyme Pharma Corp.) 相关的药物交易

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适应症	最高研发状态	国家/地区	公司	日期
冠状动脉闭塞	临床2期	加拿大	Matrizyme Pharma Corp.	2014-04-01
冠状动脉闭塞	临床2期	荷兰	Matrizyme Pharma Corp.	2014-04-01
冠状动脉闭塞	临床2期	以色列	Matrizyme Pharma Corp.	2014-04-01

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研究	分期	人群特征	评价人数	分组	结果	评价	发布日期


AUA2023	N/A	非肌层浸润性膀胱肿瘤辅助 tumor necrosis factor α (TNFα) \| interleukin (IL)-6	-	PPD injection + BCG therapy	繭糧製製選蓋膚壓蓋壓(築膚襯鹽蓋艱遞範構網) = 糧艱憲糧網艱構範遞鏇艱簾鹹鹽積遞觸構壓遞 (醖鏇築廠鹹網願築簾遞 )	-	2023-04-01
				BCG therapy alone	繭糧製製選蓋膚壓蓋壓(築膚襯鹽蓋艱遞範構網) = 鏇淵遞齋鑰衊繭構選壓艱簾鹹鹽積遞觸構壓遞 (醖鏇築廠鹹網願築簾遞 )