4-Dihydro-4-deoxy-4(R)-aminospectinomycin (I) and 4-dihydro-4-deoxy-4(S)-aminospectinomycin (II) were prepared (1) from spectinomycin via conversion to 4-O-benzyloxime followed by hydrogenation over PtO2 or (2) from 6,8-bis(N-benzyloxycarbonyl)spectinomycin via reductive amination with NaBH3CN and NH4NO3 followed by hydrogenolysis.I showed antibacterial activity comparable to that of spectinomycin; II was inactive.