作者: Liu, Yixiang ; Gong, Jiashou ; Huang, Huichuan ; Wang, Yuan ; Ye, Chen ; Jin, Yuanjin ; Mei, Xinyue ; Wang, Zhengqin ; Yang, Min ; Deng, Weiping ; Zhu, Shusheng ; Liu, Yanqi ; Song, Wenwen

Achieving safe utilization of heavy metal-contaminated soils without compromising crop productivity represents a grand challenge for sustainable agriculture. Although foliar titanium (Ti) and soil organic fertilizers (OF) independently alleviate abiotic stress, it remains unclear whether and how aboveground Ti signaling coordinates with OF mediated rhizosphere processes to enhance crop productivity and restrict heavy metal accumulation through plant-metabolite-microbiome networks. Here, using Panax notoginseng as a model, the results showed that co-application of foliar Ti with soil OF significantly outperformed individual applications. This "top-down" strategy synergistically increased root biomass and saponin content (synergistic index > 1), while reducing root Cd and Pb concentrations by 46.60% and 47.23%, respectively. Mechanistically, soil OF established a functional rhizosphere foundation by enhancing nutrient bioavailability and enriching beneficial microbial taxa. Concurrently foliar Ti acted as a systemic trigger to reprogram plant metabolism, upregulating arginine and proline metabolic pathways in leaves and coinciding with enhanced diterpenoid biosynthesis in roots. Specifically, accumulated diterpenoids (forskolin and ingenol) functioned as selective semiochemicals, recruiting a specialized microbiome consortium with enhanced metal-resistant, growth-promoting, and nutrient-solubilizing bacteria (Sphingomonas, Rhodanobacter, Bacillus) and fungi (Penicillium, Aspergillus), while inhibiting the pathogen Ilyonectria. Consequently, this engineered rhizosphere microbiome may have contributed to rhizosphere-level Cd/Pb exclusion or immobilization, thereby decoupling root metal uptake from bulk soil metal pools. This study supports a novel "aboveground trigger-belowground support" framework in which Ti-induced metabolites promote the assembly of functional microbiomes that are further supported by OF. We propose a targeted biofortification strategy leveraging plant-metabolite-microbiome coordination for safe cultivation of crops.

2026-03-01·PHYTOTHERAPY RESEARCH

Ingenol‐Mediated SIRT1 ‐ LXRα Signaling Reduces Lipid Accumulation and Alleviates Postmenopausal Liver Damage

Article

作者: Wang, Xiaogang ; Yao, Jiawei ; Liu, Meijing ; Li, Shuang

ABSTRACT:

Postmenopausal metabolic syndrome and its associated liver injury have attracted considerable research interest, yet their underlying mechanisms and treatment strategies remain insufficiently elucidated. This study aimed to investigate the relationship between aberrant lipid metabolism and hepatic injury in ovariectomized (OVX) females and to evaluate the therapeutic potential of ingenol (Ing), a natural diterpenoid, via the SIRT1–LXRα signaling pathway. Data from 3047 females in NHANES (2017–2020) were analyzed to compare serum triglyceride (TG) and liver injury markers between OVX and non‐OVX women. An OVX mouse model was established to examine hepatic lipid metabolism and SIRT1 expression. Molecular docking, dual luciferase assays, and SIRT1 silencing were performed to evaluate Ing‐SIRT1 binding and regulation. HepG2 cells were used to assess Ing's effects on lipid levels and expression of LXRα, CYP39A1, CPT1, and ACOX1. In vivo studies in OVX mice confirmed the therapeutic effects of Ing and further investigated its mechanism via the SIRT1‐LXRα pathway. NHANES data indicated that OVX women had significantly higher serum TG levels and more severe liver injury. OVX mice exhibited downregulated SIRT1 expression and disrupted lipid homeostasis. Ing showed high binding affinity to SIRT1, outperforming several known agonists. In HepG2 cells, Ing reduced intracellular TG and total cholesterol (TC), while upregulating LXRα, CYP39A1, CPT1, and ACOX1. In OVX mice, Ing treatment notably attenuated weight gain, reduced TG and TC levels, and ameliorated liver histopathological damage. These effects were mediated through the SIRT1‐LXRα pathway. Ing effectively mitigates OVX‐induced liver injury by activating SIRT1 and modulating downstream LXRα‐mediated lipid metabolic pathways. These results support Ing as a promising therapeutic candidate for liver injury in postmenopausal or OVX women.

2025-08-01·BIORESOURCE TECHNOLOGY

From waste to value: Multi-omics reveal pomegranate peel addition improves corn silage antioxidant activity and reduces methane and nitrogen losses

Article

作者: Zhang, Huixian ; Zhang, Yuanqing ; Zhang, Xia ; Wang, Dongcai

Fermentation technology presents promising opportunities for food waste valorization. Pomegranate peel (PP), a food by-product, has potential applications in fermented feed. This study examined the effects of a 6% dry PP additive on the ensiling characteristics, antioxidant activity, metabolites, bacterial community, and in vitro ruminal fermentation, methane (CH₄) emission of corn silage ensiled for 7 days and 60 days using microbiome and metabolome analyses. PP-treated silage inhibited (P < 0.05) protein degradation by reducing ammonia nitrogen and non-protein nitrogen concentrations during ensiling. The PP additive increased (P < 0.05) water-soluble carbohydrate content and reduced ethanol production in corn silage. Lactiplantibacillus dominated PP-treated silage at the initial ensiling stage, while Levilactobacillus prevailed at the final stage. Notably, the PP additive exhibited strong antioxidant activity by modulating antioxidant enzymes and flavonoid biosynthesis mediated by key metabolites (epigallocatechin and catechin). Correlation analysis identified Lactiplantibacillus, Citrobacter, Phytobacter and Burkholderia as key microbes in the production of antioxidant metabolites and enzymes in PP-treated silage. Additionally, PP supplementation reduced (P < 0.05) in vitro ruminal CH₄ and nitrogen losses, while decreasing dry matter (DM) digestibility in corn silage. In summary, PP-treated corn silage enhanced antioxidant properties and reduced the nitrogen losses and in vitro ruminal CH₄ emissions, but lowered DM digestibility. Thus, PP can be recommended as a silage additive, though the dry PP level should be lower than that used in this study.

100 项与 Ingenol 相关的药物交易

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