Triazine-based materials have attracted significant interest as chemosensors due to their diverse applications across various sectors, including the environmental monitoring, food industry, agriculture, and healthcare.The development of fluorescent chemosensors was accomplished through the synthesis of 2-aminobenzothiazole and triazine (TABTTZ).This synthesis employed multiple techniques, yielding a novel fluorescent probe derived from triazine.The probe′s selectivity for Cu2+ ions was thoroughly examined using both colorimetric and fluorescence methodologies.Selectivity assessments involving various metal ions demonstrated a pronounced affinity for Cu2+, evidenced by a color change from yellow to brown, which is attributed to the formation of a Cu2+ complex.The sensing capabilities were validated through UV spectral anal., which identified an isobestic point at 325 nm, and corroborated by Job′s plot anal. indicating a stoichiometric ratio of 1:1.Sensitivity tests for TABTTZ revealed a maximum detection limit of 0.05 μM.The presence of Cu2+ ions resulted in fluorescence quenching at 540 nm, highlighting the probe′s fluorogenic properties.The stability of both the metal complex and the ligand was further substantiated by thermogravimetric anal. (TGA).The energy difference between TABTTZ and the metal complex was assessed using d. functional theory (DFT).The viability of cells was effectively evaluated through the MTT assay, and the probe was successfully applied for biosensing in living L929 cells.Mol. docking studies indicated that the binding energy of the ligand surpassed that of the metal complex.Furthermore, antimicrobial investigations revealed that the complex demonstrated more effective inhibition compared to the probe.This study suggests that the developed chemosensor has promising potential for applications in the remediation of contaminated water and intracellular biol. environments.
