To observe the impact of the wheat germ oil and natural vitamin E soft capsule on the relieving fatigue of mice, one hundred sixty male mice were randomly divided into four groups: control group, wheat germ oil and natural vitamin E soft capsule low, middle and high dose groups.All mice were continuously intragastric administrated the soft capsule at the dosages of 0.183 g/kg·bw, 0.367 g/kg·bw, 1.100 g/kg·bw, resp. for 30 days.The loaded-swimming time, blood lactic acid, serum urea and liver glycogen were recorded.Compared with the model group, the wheat germ oil and natural vitamin E soft capsule (1.100 g/kg·bw) could remarkably increase loaded-swimming time and liver glycogen, decrease serum urea level after exertion.The results indicated that high dose group and control group difference were significant.The wheat germ oil and natural vitamin E soft capsule could relieve fatigue of the mice.