To observe the impact of the wheat germ oil and natural vitamin E soft capsule on the relieving fatigue of mice, one hundred sixty male mice were randomly divided into four groups: control group, wheat germ oil and natural vitamin E soft capsule low, middle and high dose groups. All mice were continuously intragastric administrated the soft capsule at the dosages of 0.183 g/kg·bw, 0.367 g/kg·bw, 1.100 g/kg·bw, resp. for 30 days. The loaded-swimming time, blood lactic acid, serum urea and liver glycogen were recorded. Compared with the model group, the wheat germ oil and natural vitamin E soft capsule (1.100 g/kg·bw) could remarkably increase loaded-swimming time and liver glycogen, decrease serum urea level after exertion. The results indicated that high dose group and control group difference were significant. The wheat germ oil and natural vitamin E soft capsule could relieve fatigue of the mice.