4区 · 工程技术
ArticleOA
作者: Papanastasiou, Malvina ; Bou-Assaf, George M. ; Rouse, Jason C. ; Weis, David D. ; Brock, Ansgar ; Sperry, Justin B. ; Srinivasan Anand, Ganesh ; Li, Sheng ; Deredge, Daniel ; Rand, Kasper D. ; Carroll, James A. ; Lu, Xiaojun ; Zhang, Hui-Min ; Anderson, Kyle W. ; Lambris, John D. ; Gallagher, Elyssia S. ; Zhang, Jennifer ; Chen, Guodong ; Steckler, Caitlin ; Urata, Sarah ; Huang, Richard Y.-C. ; Park, Inhee ; Hageman, Tyler S. ; Karageorgos, Ioannis ; Harguindey, Eduardo ; Chandramohan, Arun ; Venable, John ; Walters, Benjamin T. ; Al-Naqshabandi, Mohammed A. ; Wintrode, Patrick L. ; Chalmers, Michael J. ; Pandey, Ratnesh ; Nirudodhi, Sasidhar N. ; Hudgens, Jeffrey W. ; Espada, Alfonso ; Leurs, Ulrike
The spreadsheet file reported herein provides centroid data, descriptive of
deuterium uptake, for the FabFragment of NISTmAb (PDB: 5K8A) reference material, as
measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS)
method. The protein sample was incubated in deuterium-rich solutions under uniform
pH and salt concentrations between 3.6 oC and 25.4 oC for seven intervals ranging
over (0 to 14,400) s plus a ∞pseudo s control. The deuterium content of peptic
peptide fragments were measured by mass spectrometry. These data were reported by
fifteen laboratories, which conducted the measurements using orbitrap and Q-TOF mass
spectrometers. The cohort reported ≈ 78,900 centroids for 430 proteolytic peptide
sequences of the heavy and light chains of NISTmAb, providing nearly 100 % coverage.
In addition, some groups reported ≈ 10,900 centroid measurements for 77 peptide
sequences of the Fc fragment. The instrumentation and physical and chemical
conditions under which these data were acquired are documented.
