A review, with 21 references, is given on the application of sephadex LH-20 to the separation and purification of glycosides, the major kind of active constituents from natural products, attracting attention for their wide distribution and particular pharmacol. activities, and including flavonoid glycosides, triterpenoid saponins, lignan glycosides, phenylethanoid glycosides, cardiac glycosides, and iridoid glycoside.