This study showed the complexity of quantifying target analytes within antigen arrays.Production of purified antibodies is laborious and expensive.Methods that can be used for antibody purification, e.g., affinity chromatog., could theor. be used to obtain material comparable to the target analyte of an antigen array.However, consistent antibody purity is paramount for quantification.Although this approach has broad application for the comparison of any IgG, it will not measure absolute concentrations of target analyte.This is largely because of the presence of factors in sera that can interfere with antibody-based immunoassays.Nonetheless, this method has reduced intraassay variability and enables interassay comparisons for a wide range of antigen arrays.