Objective To establish the methods of determining the contents of evodiamine, rutacarpine and limonin in Evodia rutaecarpa by HPLC simultaneously. Method Agilent 5 TC-C18 (2) (250 nm×4.6 nm, 5 μm) chromatog. column was adopted, the mobile phase was acetonitrile-aqueous solution (53: 47, V/V), the same degree of elution, the detection wavelength was 215 nm, the flow rate of mobile phase was 1 mL/min, and the column temperature of chromatog. column was 25°C. Result The separation of evodiamine, rutaecarpin and limonin was good. The three components were linear in the corresponding range; the average recovery of evodiamine, rutaecarpin and limonin was 98.0%, 97.8%, 97.3% (n=6), resp. Conclusion The method is simple, accurate and reproducible, and is suitable for the quality control of Evodia rutaecarpa.