In order to evaluate the application effect of potassium persulfate composite powder in chicken house, the disinfection effect evaluation test of potassium persulfate composite powder on natural bacteria in the chicken house, the cleaning test of water pipeline in the chicken house and the air disinfection test were carried out. The results showed that when the potassium persulfate complex powder was diluted by 1:400 times, it could effectively kill the natural bacteria in the chicken house. When the potassium persulfate complex powder was diluted by 1:200 times, it had a better cleaning effect on the drinking water pipeline. When the potassium persulfate complex powder was diluted by 1:100 times and disinfected for 1 h, the sterilization rate of natural bacteria in the air of chicken house reached 100%.