Lactic acid pediocin can be used as a preservative to extend the shelf life of food.The full gene sequence of Ped-A and without signal peptide were inserted into expression vectors pMG36e and pNZ8048.By using restriction enzyme and sequence anal., it can be proved that recombinant plasmids pMG36e/Ped-A (full length/fragment) and pNZ8048/Ped-A (full length/fragment) were transformed into E. coli DH5α and DHB4 successfully.The result confirmed that Ped-A gene expression vectors were constructed successfully.