Objective To identify the effective components and determine the content of glucosamine hydrochloride in Jingu capsule.Methods Chem. reaction, thin layer chromatog.(TLC) and high performance liquid chromatog.(HPLC) were used for the effective components identification and determination of glucosamine hydrochloride content.Results Red Cu20 precipitate was formed by the reaction of sample and glucosamine hydrochloride with copper tartrate test solution, white flocculent AgCl precipitate was formed by the reaction with silver nitrate test solution, blue purple solution was formed by the reaction with ninhydrin test solution, and dark red spots were formed at corresponding positions of TLC.The sample and paeoniflorin reference substance generated blue-purple spots on the corresponding positions of TLC.The sample and calcium carbonate reference reacted with dilute hydrochloric acid test solution to generate CO_2 gas and reacted with ammonium oxalate test solution to generate white CaC_2 O_4 precipitateThe retention time of HPLC peaks of the sample and vitamin K_1 reference was the same as the sample and vitamin D3 referenceNone of the neg. samples had the above reaction.At the same time, the content of glucosamine hydrochloride in the sample was determined by HPLC.The linear relation was good with the linear coefficient r∼2 0.9999 in the linear range of 96∼1200 μg/mL.The limit of detection was 1.2μg/mL, the relative standard deviation(RSD) was 0.72%, and the recovery rates were 99.78%∼100.40%.The glucosamine hydrochloride content of 3 consecutive batches of samples was determined and the results were 64.94 g/100 g, 64.25 g/100 g and 65.12 g/100 g, resp., with the RSD 0.48%.Conclusion These methods of identification and content determination have strong specificity, good reproducibility and high sensitivity, and could be used to control the product quality, which is suitable for the identification of effective components and determination of glucosamine hydrochloride content in Jingu capsule.