This paper established a method for the determination of ginsenoside Rg1 in Jiangtangning capsules.Ginsenoside Rg1 was determined by a dual-wavelength TLC scanning method with 525 nm and 700 nm as the detective wavelength.The results showed that there was a good linear relationship from 0.323 to 2.584 μg for ginsenoside Rg1 (r = 0.9995), and the average recovery rate was 98.06% (RSD = 1.33%, n = 5).In conclusion, the method is simple and accurate, and can be used for the quality control of Jiangtangning capsules.