Objective: To provide scientific evidence for establishing research method on quality standard of Xugu oil.Methods: Rhubarb, lignum acronychiae, flos Rhododendri Mollis in Xugu oil were identified by thin layer chromatog. (TLC).Limit test for total alkaloid content in diester diterpenoid alkaloids (DDAs) (aconitine, hypaconitine, mesaconitine) and content of strychnine by high performance liquid chromatog. (HPLC).Detection mobile phase A of DDAs was acetonitrile:tetrahydrofuran (5:3), mobile phase B was 0.1 mol/L ammonium acetic solution (add glacial acetic acid 0.5 mL per 1000 mL), detection wavelength was 238 nm.Detection mobile phase A of Strychnine was acetonitrile, mobile phase B was equivalent mixed solution of 0.01 mol/L sodium heptanesulfonate and 0.02 mol/L potassium dihydrogen phosphate (adjust the pH value to 2.8 with 10% phosphoric acid), detection wavelength was 260 nm.Results: Differentiation method of rhubarb, lignum acronychiae and flos Rhododendri Mollis were specific, average total alkaloid content of DDAs were 0.0110μg/mL, average content of strychnine was 0.6206μg/mL, did not exceed the specified requirements.Conclusion: Method for examination in the research is a simple, specific method with good reproducibility.It can provide scientific evidence for establishing quality standard of Xugu oil.

Guangdong Yixue

Effect of ulinastatin on the development of traumatic coagulation disease to multiple organ dysfunction syndrome

作者: Wang, Guang-wei ; Lu, Yan-yi ; Liu, Yu-mei ; Huo, Li-wei ; Liu, Yun-song ; Zhang, Chi ; Tan, Yu ; Li, Yun-feng ; He, Hua-yong

Objective To observe the protective effect of ulinastatin on the occurrence and development of multiple organ dysfunction syndrome (MODS) in patients with traumatic coagulation disease.Methods 80 patients who met the diagnostic criteria of traumatic coagulation disease were randomly divided into observation group and control group.The patients in both groups were treated according to the routine treatment of traumatic coagulation disease.The observation group was added with ulinastatin 400000 U i.v. drip, twice a day.The prothrombin time (PT), activated partial thromboplastin time (APTT), platelet count (PLT), fibrinogen content (FIB) and D-dimer content (D-D) were dynamically observed before treatment and on the 1st, 2nd, 3rd, 5th, 7th, 10th and 14th days after treatment.The levels of interleukin-6 (IL-6) and interleukin-10 (IL-10) in the blood of the two groups were measured before treatment and on the 1st, 2nd, 3rd, 5th, 7th, 10th and 14th days after treatment.The changes of Apache II score, ISS score and MARSHALL score were recorded before treatment and on the 1st, 2nd, 3rd, 5th, 7th, 10th and 14th days after treatment.The length of stay in ICU, the incidence of MODS and 30 day mortality were compared between the two groups.Results there was no significant difference between the two groups before treatment (P>0.05).Pt, APTT, PLT, FIB and D-D in the observation group were lower than those in the control group on the 1st, 2nd, 3rd, 5th, 7th, 10th and 14th days after treatment (P<0.05).The contents of inflammatory mediators IL-6 and IL-10 in the observation group were significantly lower than those in the control group on days 1, 2, 3, 5, 7, 10 and 14, and returned to normal earlier than those in the control group (days 7 and 10, resp.).There was significant difference between the two groups (P<0.05).The Apache II score, ISS score and MARSHALL score of the observation group were better than those of the control group on the 1st, 2nd, 3rd, 5th, 7th, 10th and 14th days after treatment (P<0.05).The ICU length of stay, the incidence of MODS and the 30 day mortality in the observation group were significantly lower than those in the control group (P<0.05).Conclusion early addition of Ulinastatin to patients with traumatic coagulation disease on the basis of routine treatment can significantly improve the coagulation status, reduce the degree of inflammatory reaction, shorten the length of stay in ICU, reduce the incidence of MODS and 30 day mortality, and improve the prognosis of patients.

Xiandai Zhenduan Yu Zhiliao

Correlation between knee osteoarthritis and C-reactive protein and erythrocyte sedimentation rate

作者: Xu, Yan-qun ; Wei, Rong-mei ; Wu, Hong-hui

Objective To analyze the correlation between knee osteoarthritis (KOA), C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR).Methods 80 KOA patients treated in our hospital from Jan. 2018 to Sept. 2018 were selected, the clin. data of 38 patients in remission stage were divided into control group, and the clin. data of 42 patients in active disease stage were divided into two groups. observation group.The levels of ESR and CRP in the two groups were observed and compared, and the correlation between KOA activity and ESR and CRP was analyzed.Results The levels of ESR and CRP in the observation group were significantly higher than those in the control group, and the difference was statistically significant (P<0.05); The Pearson correlation test showed that KOA disease activity was pos. correlated with CRP and ESR (P<0.05).Conclusion There is a correlation between the KOA activity level and ESR and CRP, By measuring the levels of ESR and CRP, the severity of the patient′s disease can be assessed and clin. treatment can be guided.

100 项与泉州市正骨医院相关的药物交易

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100 项与泉州市正骨医院相关的转化医学

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