作者: Chanda, Nripen ; Upendran, Anandhi ; Kannan, Raghuraman ; Zambre, Ajit ; Prayaga, Sudhirdas ; Afrasiabi, Zahra ; Almudhafar, Rosana
We have successfully developed gold nanoparticle based immunostrip assay to detect protein-A (PA). Rabbit polyclonal antibody IGg (αPA) that has affinity to PA was conjugated to gold nanoparticles (GNPs) and the gold nanoconjugate (αPA-GNP) was used to detect protein-A by simple immunostrip assay method. ELISA experiments were used to confirm the retention of binding affinity of antibody towards protein-A after conjugation with gold nanoparticles.
