Objective To investigate the stability of cefepime hydrochloride injection and 5-hydroxymethyfurfura (5-HMF) in 2 commonly used infusion in clinic by HPLC. Methods The HPLC method fordetn. of cefepime dihydrochloride injection and 2 injections in their mixed solutions were established, and the stability of 5-hydroxymethyfurfura were assayed in the mixture during 8 h. By C18 column chromatog., the mobile phase consisted of methanol and pure water (10:90). The flow rate was 1.0 mL/min and detection wavelength was 284 nm. Results The mass concentration of 5-hydroxymethyfurfura in the range of 0.512 5-20.50 μg/mL showed good linear relation with the peak area. Cefepime Hydrochloride Injection was mixed in 10% glucose injection and 5% glucose sodium chloride injection, the average recovery was 99.94% (RSD = 1.23%) and 99.68% (RSD = 1.51%), resp. Conclusion The method is simple, accurate, reproducible, and can be used for the quality control of 5-hydroxymethyfurfura in cefepime hydrochloride for injection mixed with two kinds of infusions.