PURPOSEWe describe the design and application of a new apparatus for applying Radiofrequency (RF) electromagnetic fields to cells in culture on a microscope stage. This new design enables real-time studies of the actuation of magnetic nanoparticles bound to membrane receptors or internalised within cells together with the study of magnetic fluid hyperthermia (MFH)-associated effects.MATERIALS AND METHODSRF coils were fabricated and electromagnetic simulations were performed along with compatibility evaluations and calorimetric experiments using this apparatus at discreet frequencies between 100 kHz and 1 MHz. Cell killing via MFH was investigated in a neuroblastoma tumour cell line.RESULTSSimulations and evaluations showed that the field intensity and homogeneity experienced by the cells within the chamber is best with a planar coil configuration. The incubation chamber was suitable for cell culture and the design was compatible with mountings on different makes of microscopes as it mimics a standard 96/24/6 tissue-culture well plate. Successful calorimetric and MFH cytotoxicity proof-of-principle experiments were performed and are presented.CONCLUSIONSWe conclude from these experiments that alternating magnetic field (AMF)-mediated activation and magnetic fluid hyperthermia (MFH) research will benefit from this RF coil that fits inside an incubation chamber, mounted onto a microscope. This new design could be used to assist real-time MFH studies in vitro.

2014-03-01·Journal of Tissue Engineering and Regenerative Medicine2区 · 工程技术

Efficient transfection of MG-63 osteoblasts using magnetic nanoparticles and oscillating magnetic fields

2区 · 工程技术

Article

作者: Farrow, N. ; Dobson, J. ; Fouriki, A. ; Clements, M.A.

AIMSTo examine the potential of magnetic nanoparticles (MNPs) in transfecting human osteosarcoma fibroblasts (MG-63) and investigate the effects of a novel non-viral oscillating nanomagnetic gene transfection system (magnefect-nano™) in enhancing transfection efficiency (TE).METHODSMG-63 cells were transfected using MNPs coupled with a GFP-carrying plasmid. The magnefect-nano system was evaluated for transfection efficiency and potential associated effects on cell viability.RESULTSMG-63 cells were efficiently transfected using MNPs and the magnefect-nano system significantly enhanced overall transfection efficiency. MNPs were not found to affect cell viability and/or function of the cells.CONCLUSIONNon-viral transfection using MNPs and the magnefect-nano system can be used to transfect MG-63 cells and assist reporter gene delivery on a single cell basis, highlighting the wide potential of nanomagnetic gene transfection in gene therapy.

2010-01-01·Nano Reviews

Evaluation of the magnetic field requirements for nanomagnetic gene transfection

Article

作者: Farrow, N. ; Clements, M.A. ; Fouriki, A. ; Dobson, J.

The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency.METHODSnon-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays.RESULTSFluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically.CONCLUSIONIn all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000™.

100 项与 nanoTherics Ltd. 相关的药物交易

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