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100 项与 Ludwik Hirszfeld Institute Of Immunology & Experimental Therapy 相关的临床结果

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项与 Ludwik Hirszfeld Institute Of Immunology & Experimental Therapy 相关的文献（医药）

2024-10-01·Biomedicine & Pharmacotherapy

Calcium ion delivery by microbubble-assisted sonoporation stimulates cell death in human gastrointestinal cancer cells

Article

作者: Baczyńska, Dagmara ; Kulbacka, Julita ; Przystupski, Dawid ; Ussowicz, Marek ; Rossowska, Joanna

Ultrasound-mediated cell membrane permeabilization - sonoporation, enhances drug delivery directly to tumor sites while reducing systemic side effects. The potential of ultrasound to augment intracellular calcium uptake - a critical regulator of cell death and proliferation - offers innovative alternative to conventional chemotherapy. However, calcium therapeutic applications remain underexplored in sonoporation studies. This research provides a comprehensive analysis of calcium sonoporation (CaSP), which combines ultrasound treatment with calcium ions and SonoVue microbubbles, on gastrointestinal cancer cells LoVo and HPAF-II. Initially, optimal sonoporation parameters were determined: an acoustic wave of 1 MHz frequency with a 50 % duty cycle at intensity of 2 W/cm². Subsequently, various cellular bioeffects, such as viability, oxidative stress, metabolism, mitochondrial function, proliferation, and cell death, were assessed following CaSP treatment. CaSP significantly impaired cancer cell function by inducing oxidative and metabolic stress, evidenced by increased mitochondrial depolarization, decreased ATP levels, and elevated glucose uptake in a Ca²⁺ dose-dependent manner, leading to activation of the intrinsic apoptotic pathway. Cellular response to CaSP depended on the TP53 gene's mutational status: colon cancer cells were more susceptible to CaSP-induced apoptosis and G1 phase cell cycle arrest, whereas pancreatic cancer cells showed a higher necrotic response and G2 cell cycle arrest. These promising results encourage future research to optimize sonoporation parameters for clinical use, investigate synergistic effects with existing treatments, and assess long-term safety and efficacy in vivo. Our study highlights CaSP's clinical potential for improved safety and efficacy in cancer therapy, offering significant implications for the pharmaceutical and biomedical fields.

2024-09-25·Nanomedicine

Antitumoral melatonin-loaded nanostructured lipid carriers

Article

作者: Bonilla-Vidal, Lorena ; Espina, Marta ; García, Maria Luisa ; Świtalska, Marta ; Wietrzyk, Joanna ; Souto, Eliana B ; Gliszczyńska, Anna ; Sánchez-López, Elena

Aim: Cancer constitutes the second leading cause of death worldwide, with conventional therapies limited by significant side effects. Melatonin (MEL), a natural compound with antitumoral properties, suffers from instability and low solubility. To overcome these issues, MEL was encapsulated into nanostructured lipid carriers (MEL-NLC) containing rosehip oil to enhance stability and boost its antitumoral activity.Methods: MEL-NLC were optimized by a design of experiments approach and characterized for their physicochemical properties. Stability and biopharmaceutical behavior were assessed, along with interaction studies and in vitro antitumoral efficacy against various cancer cell lines.Results: Optimized MEL-NLC exhibited desirable physicochemical characteristics, including small particle size and sustained MEL release, along with long-term stability. In vitro studies demonstrated that MEL-NLC selectively induced cytotoxicity in several cancer cell lines while sparing healthy cells.Conclusion: MEL-NLC represent a promising alternative for cancer, combining enhanced stability and targeted antitumoral activity, potentially overcoming the limitations of conventional treatments.

2024-09-01·Carbohydrate Research

Structure of the O-specific polysaccharide of Asaia bogorensis ATCC BAA-21 lipopolysaccharide

Article

作者: Kasperkiewicz, Katarzyna ; Noszczyńska, Magdalena ; Kaczmarek, Anna ; Maciejewska, Anna ; Łukasiewicz, Jolanta

Asaia bogorensis is a Gram-negative bacterium isolated from flowers and fruits growing in tropical climate, reproductive system of mosquitoes, and rarely from immunocompromised patients. In Europe, A. bogorensis is responsible for the contamination of flavoured mineral waters. One of the important surface antigen and an element of the bacterial biofilm is lipopolysaccharide (LPS, endotoxin). To date, no data on A. bogorensis LPS structure has been reported. Chemical analysis and ¹H,¹³C nuclear magnetic resonance spectroscopy revealed the novel structure of the O-specific polysaccharide of A. bogorensis ATCC BAA-21 LPS. It was concluded that the repeating unit of the O-antigen is a branched trisaccharide with the following structure: →6)-α-d-Glcp-(1→2)-[β-d-Glcp-(1→3)]-α-l-Rhap-(1→ .

100 项与 Ludwik Hirszfeld Institute Of Immunology & Experimental Therapy 相关的药物交易

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100 项与 Ludwik Hirszfeld Institute Of Immunology & Experimental Therapy 相关的转化医学

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