The alfalfa saponin was separated from alfalfa by macroporous resin. The alfalfa saponin was used in the manufacture of decreasing blood fat, anti-atherosclerosis drug, and other features of health care. Using the perennial herb-alfalfa for raw materials, according to the raw materials: solvent = 1:8 ratio by adding 45% ethanol solvent, temperature 80°, for two times, the special macroporous resin adsorption water impurity, and then by 30% and 75% ethanol solvent gradient elution, then final recovery of ethanol, spray drying, alfalfa saponin products was obtained. After 20 consecutive granted normal preprodn., the average recovery rate of alfalfa saponin was 93.8%, the product of alfalfa saponin content in an average was 80.4%, the average yield of raw materials was 1.72%. The test proved that the production of this process was successful, it would improve product in alfalfa saponin content to more than 80% at one-time, and it could be used in the pharmaceutical industry to promote the new technol.