Shanghai Claison Bio-Tech Co., Ltd.

The aim of the present study was to investigate the biological features of in vitro cultured γδ T cells. The γδ T cells were in vitro cultured and on different culture days cell proliferation, phenotype, killing activity and the secretion of cytokines were analyzed. Cell numbers were counted by an automated cell counter, phenotype of the cells and cytokines were analyzed by flow cytometry, and killing activities of the cells against gastric cancer SGC-7901 cells were tested using the cell counting kit-8. From days 7 to 14, in vitro cultured γδ T cells enter the exponential phase. On day 14, maximum proliferation fold was observed, and on day 10, the maximum specific growth rate µmax was achieved. Flow phenotype cluster of differentiation 3⁺-T-cell receptor γδ⁺ of the γδ T cells in the first 7-17 days achieved a higher proportion and showed no significant differences between 10 days. Secretion of the cytokines interferon-γ and tumor necrosis factor-α gradually increased in the first 7-14 days. The maximum was achieved on day 14, and subsequently began to decrease. The cytolytic activity of the γδ T cells to kill the SGC-7901 cells in the first 7-14 days had an improved killing effect, a slight decline from the first 17 days; in the effector cell to target cell (E:T) ratio 20:1, 10:1 and 5:1 conditions, γδ T cells kill SGC-7901 cells more effectively than 1:1 and 1:2. In conclusion, γδ T cells cultured in the first 7-14 days are suitable for clinical transfusion, and the optimal transfusion time is day 10. An E:T ratio >5:1 is preferred.

Undifferentiated embryonal sarcoma of the liver (UESL) is a rare malignant tumor that occurs predominantly in children and has a poor prognosis. Here, we report a novel case in which the UESL presented in the left lobe of the liver and metastasized into both lungs after surgical resection. The patient recovered after our administration of an immunotherapeutic combination of DCs (Dendritic Cells) and multi-immune cells, such as cytokine-induced killer cells (CIKs) and natural killer cells (NKs). After the third cycle of immunotherapy, a CT scan showed a remarkable regression of the lung metastases. This finding supports the conclusion that the DC-based treatment is a promising potential strategy for treating patients with relapsed UESL.

The objective of this study was to observe the effects of polysaccharides in Inonotus obliquus on differential gene expression profiling in mouse infected with Toxoplasma gondii and discuss the mechanism of inhibiting Toxoplasma gondii.BABL/C mice were inoculated i.p. with 103 tachyzoites of Toxoplasma gondii of RH strain to establish a mouse model of infection of toxoplasmosis.The mice were randomly divided into three groups: normal control group, neg. control group and Inonotus obliquus polysaccharide group.Animal liver were collected and frozen-stored quickly.Its RNA was extracted and amplified, then hybridization was conducted.The figures were scanned for data anal.The effective differential genes of polysaccharide group were searched out by SOM anal.The effective differential genes were annotated on gene functional aspect.The main reasons of which Inonotus obliquus polysaccharide inhibited Toxoplasma were announced.The mechanism that the effects of polysaccharides in Inonotus obliquus inhibited Toxoplasma gondii are achieved by effectively regulating the cytokine levels of mice, promoting Th1/Th2 balance, controlling Toll-like receptor signaling pathways and integral controlling the body.