Shenzhen Zhongshan Urological Hospital

The aim of this study was to evaluate the effects of quarter zona-pellucida (ZP) opening by laser-assisted hatching (QLAH) on the clinical outcomes following transfer of vitrified-warmed blastocysts developed from low-grade cleavage-stage embryos in patients with all high-grade and fair-grade cleavage-stage embryos transferred without achieving pregnancy. Patients were randomized into two groups: QLAH (n=101) and control (n=102). The implantation and clinical pregnancy rates were significantly higher in the QLAH group compared with the control group (P=0.021 and P=0.034, respectively). The live birth rate of the QLAH group was also higher, although not significantly. When the clinical outcomes according to the day of blastocyst vitrification were compared between the groups, the implantation, clinical pregnancy and live birth rates of the QLAH group were significantly higher (P<0.05) than those of the control group for day 6 blastocysts, but not for day 5 or day 5/day 6 blastocysts. These results suggest that QLAH improves the clinical outcomes of vitrified-warmed blastocysts, especially of day 6 vitrified blastocysts, developed from low-grade cleavage-stage embryos.

To search for a simple, quick and accurate method for the sperm DNA integrity test by modifying the conventional sperm chromatin dispersion (SCD) test, and to investigate the reference value range of normal sperm DNA integrity by the improved SCD assay.

We modified the conventional SCD test in its procedures, reagents preparing and utensils to be used, compared the improved method with the conventional one, and detected 293 normal semen samples for sperm DNA integrity by the modified SCD test.

An improved SCD test was established, and no statistical differences were found between the results of the modified and conventional methods. The reference value range was determined by the improved SCD assay for normal sperm DNA integrity, which was < 32.58% for abnormal sperm DNA integrity.

The improved SCD test could detect sperm DNA integrity more quickly, and it reduced the cost in reagents.

Objective To investigate the pregnancy outcome and influencing factors of patients with low ovarian response (POR) in the follow-up treatment cycle without ovulation.Methods Retrospective anal. was made on the clin. data of the cycles of unfertilized eggs and subsequent treatment cycles of POR patients admitted to the center from Jan. 1, 2014 to Dec. 31, 2017, and the pregnancy outcome and its influencing factors in the cycles of subsequent treatment of unfertilized eggs were analyzed T test or X², Carry out statistical anal.Results There were 186 cycles of unfertilized eggs in POR patients, with an incidence of 8.19%.Among the patients without eggs, 159 patients had 339 cycles of egg retrieval, and 39 patients had live births.The age of patients with live birth was significantly lower than that of patients without live birth (P<0.05).There was no significant difference in AMH, basal FSH, AFC and BMI between the two groups (P>0.05).After 159 patients were divided into 4 groups:<35 years old, 35-38 years old, 39-42 years old and>42 years old, the implantation rate, clin. pregnancy rate and live birth rate decreased significantly with age, and the early abortion rate increased significantly with age (P<0.05).Conclusion In the course of assisted reproductive therapy, some patients with POR can still obtain live birth even though they have experienced a cycle of unfertilized eggs.Age is the most valuable predictor of clin. outcome in the follow-up treatment cycle.