The aim is to set up a method for determining Specnuezhenide in Shengxinfa Capsules. With HPLC method, Agilent TC-C18 column(4.6 mm*250 mm, 5 μm) was used. Mobile phase was methanol-water(35:65), the flow rate was 1.0 mL/min, and the detecting wavelength was at 224 nm. The linearity was in the range of 0.15-0.90 μg. The average recovery was 100.35%, and RSD was 1.43%. The method is accurate, reliable, and can be used for determination of Specnuezhenide in Shengxinfa Capsules for its quality control.