Purpose: This study aims to explore the application of preparative liquid chromatog. in the purification and quality enhancement process of Coenzyme Q10 (CoQ10) to improve its purity and yield, providing new insights and tech. support for the industrial production of CoQ10.Methods: In this study, we employed a novel tech. approach designed to remove impurities such as 5-demethoxy Coenzyme Q10 from CoQ10 to enhance its quality.Firstly, we prepared a mixed sample of CoQ10, including the target product and impurities.Subsequently, we utilized liquid chromatog. with specific parameters such as flow rate, column temperature, and detection wavelength to sep. the target product from impurities.The key to this method lies in the optimization of chromatog. conditions to ensure the efficient and precise separation of CoQ10.Results: Exptl. results demonstrate that the application of preparative liquid chromatog. successfully achieved the purification and quality enhancement of CoQ10.Through the optimization of chromatog. conditions, we effectively removed impurities like 5-demethoxy Coenzyme Q10, thereby increasing the purity of CoQ10.Simultaneously, this method significantly improved the yield of CoQ10, enhancing its com. value.Conclusion: This study validates the feasibility and effectiveness of preparative liquid chromatog. in CoQ10 productionThe method offers a viable approach to enhance the quality and yield of CoQ10 and provides new perspectives for its industrial productionTherefore, liquid chromatog. may serve as a powerful tool in the purification and quality enhancement process of CoQ10, potentially promoting its widespread application and market expansion.
