A rapid method for the determination of quinolones residues in milk was established by using qechers-hplc-ms.The results showed that the linearity of six quinolones was good in the range of 1.00∼50.0μg·L-1, the correlation coefficient was higher than 0.998, the recovery rate of the method was 88.2%∼106.2%, the precision was 3.9%∼9.7%, the detection limit was 0.38∼0.50μg·kg-1, and the precision was less than 10%.The method is simple, sensitive, accurate and reliable, and can meet the requirements of the detection of six quinolones in milk.