Objective: To evaluate the differences in the detection results of three different types of matrix vitamin K1, MK4 and MK7 in fingertip blood, serum and plasma, and to provide guidance and reference for medical laboratory laboratories to select sample matrix for the detection of vitamin K1, MK4 and MK7. Methods: The fingertip blood and venous blood were collected from 30 healthy volunteers, and the venous blood was made into serum and plasma resp. Vitamin K1, MK4 and MK7 in three different matrixes of fingertip blood, serum and plasma were detected by the high-performance liquid chromatog.-mass spectrometry method developed by our laboratory, and the statistical anal. was carried out. Results: There was no significant difference in the results of vitamin K1, MK4, and MK7 in three different types of matrix: fingertip blood, serum and plasma (all P > 0.05). Conclusion: When medical laboratory detects vitamin K1, MK4 and MK7, reliable detection results can be obtained by selecting fingertip blood, serum and plasma.